STUDIES ON THE STRUCTURE OF KERATIN .2. THE AMINO ACID CONTENT OF FRACTIONS ISOLATED FROM OXIDIZED WOOL
STUDIES ON THE STRUCTURE OF KERATIN .2. THE AMINO ACID CONTENT OF FRACTIONS ISOLATED FROM OXIDIZED WOOL
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DOI：
10.1016/0006-3002(56)90048-8
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发表时间：
1956-01-01
期刊：
影响因子：
--
通讯作者：
KNIGHT, CS
中科院分区：
文献类型：
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作者：
EARLAND, C;KNIGHT, CS
用纸层析法对过氧乙酸氧化羊毛部分的水解物进行了分析。结果表明，与未处理的羊毛相比，羊毛的色谱图更为复杂。因此，可溶于氨水和不溶于氨水的馏分中含有绵羊硫磺。然而，这一很弱的斑点在两个组分中具有相同的强度，并且没有证据表明在不溶性组份中存在任何其他交联键。结果表明，过氧乙酸除了氧化羊毛中所有的胱氨酸残留物外，还氧化了部分赖氨酸和精氨酸残留物。羊毛先与氰化钾溶液反应，然后与过氧乙酸反应，在水解液中生成绵羊硫磺和半胱酸。这表明氰化物溶液将胱氨酸残基转化为羊硫氨酸的反应不是定量的。
The hydrolysates of fractions isolated from wool oxidized with peracetic acid were examined by paper chroma-tography. It is shown that the chromatograms are more complex than those of untreated wool. Thus, the fractions soluble and insoluble in ammonia solution contain lanthionine sulphone. However, this spot which is very weak, is of equal intensity in the 2 fractions and no evidence for the presence of any other cross-links in the insoluble fraction could be found. It is shown that, in addition to oxidizing all the cystine residues in wool, peracetic acid oxidized some of the lysine and arginine residues. Wool, when reacted with potassium cyanide solutions followed by peracetic acid, produces on hydrolysis lanthionine sulphone and cysteic acid. This shows that conversion of the cystine residues to lanthionine by cyanide solutions is not quantitative.