Interaction of nucleoredoxin with protein phosphatase 2A
Interaction of nucleoredoxin with protein phosphatase 2A
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DOI：
10.1016/j.febslet.2006.04.101
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发表时间：
2006-06-26
期刊：
影响因子：
3.5
通讯作者：
Zolnierowicz, Stanislaw
中科院分区：
文献类型：
--
作者：
Lechward, Katarzyna;Sugajska, Ewa;Zolnierowicz, Stanislaw
用硫代磷酸化酶亲和层析法从兔骨骼肌中分离出一种由催化亚基（PP 2Ac）、结构亚基（PR 65/A）和调节亚基（PR 55/B）组成的三聚体蛋白磷酸酶2A（PP 2A（T55）），它含有两种分别为54和55 kDa的蛋白质。该54 kDa蛋白被鉴定为真核翻译终止因子1（eRF 1）和PP 2A相互作用蛋白[Andjelkovic等（1996）EMBO J.15，101-112]。55 kDa的蛋白质现在被鉴定为核氧还蛋白（NRX）。通过用纯化形式的PP 2A进行的下拉实验，以及通过在HEK 293细胞中表达的HA标记的NRX与内源性PP 2A亚基复合的免疫沉淀，证明了GST-NRX、PP 2A（C）和PP 2A（D）之间形成复合物。在GST-NRX存在下的PP 2A活性分析表明，NRX与聚阳离子竞争对不同形式的PP 2A的刺激和抑制作用。(c)2006年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
A trimeric protein phosphatase 2A (PP2A(T55)) composed of the catalytic (PP2Ac), structural (PR65/A), and regulatory (PR55/B) subunits was isolated from rabbit skeletal muscle by thiophosphorylase affinity chromatography, and contained two additional proteins of 54 and 55 kDa, respectively. The 54 kDa protein was identified as eukaryotic translation termination factor 1 (eRF1) and as a PP2A interacting protein [Andjelkovic et al. (1996) EMBO J. 15, 101-112]. The 55 kDa protein is now identified as nucleoredoxin (NRX). The formation of a complex between GST-NRX, PP2A(C) and PP2A(D) was demonstrated by pull-down experiments with purified forms of PP2A, and by immunoprecipitation of HA-tagged NRX expressed in HEK293 cells complexed endogenous PP2A subunits. Analysis of PP2A activity in the presence of GST-NRX showed that NRX competed with polycations for both stimulatory and inhibitory effects on different forms of PP2A. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.