Overlapping, but Not Identical, Sites Are Involved in the Recognition of C3bi, Neutrophil Inhibitory Factor, and Adhesive Ligands by the αMβ2 Integrin*
Overlapping, but Not Identical, Sites Are Involved in the Recognition of C3bi, Neutrophil Inhibitory Factor, and Adhesive Ligands by the αMβ2 Integrin*
复制标题
重叠但不相同的位点参与 αMβ2 整合素*对 C3bi、中性粒细胞抑制因子和粘附配体的识别
DOI：
--
复制
发表时间：
1996
影响因子：
4.8
通讯作者：
E. Plow
中科院分区：
文献类型：
--
作者：
Li Zhang;E. Plow
The αMβ2 (CD11b/CD18, Mac-1) integrin receptor binds numerous ligands, including neutrophil inhibitory factor (NIF), C3bi, and certain immobilized protein substrates, represented by denatured ovalbumin. These ligands share no obvious structural similarities, yet their interactions with receptor are inhibited by NIF and involve the I domain, a stretch of ∼200 amino acids in the αM subunit. Recombinant wild-type and mutant forms of αMβ2 have been used to compare the recognition requirements of these ligands. The various constructs were expressed efficiently on the surface of human embryonic kidney 293 cells and formed α·β heterodimeric complexes. The wild-type transfectants bound the three ligands in a similar fashion to naturally occurring αMβ2. NIF inhibited these interactions, and deletion of the D248PLGY from within the I domain abolished binding of all three ligands, suggesting an overlapping recognition specificity. A single point mutation of Ser138 to Ala in the β2 subunit abolished C3bi binding and cell adhesion but did not affect NIF binding. A switch of the R281QELNTI sequence in helix 6 of the αM I domain to the corresponding sequence in the I domain of the αL (QETLHKF) subunit completely abrogated adhesion while not affecting C3bi and NIF binding. The two mutant receptors also did not support activation-dependent adhesion to fibrinogen. Thus, the contact sites for NIF, C3bi, and adhesive proteins, represented by denatured ovalbumin and fibrinogen, in αMβ2 are overlapping but not identical.
登录
查看更多内容
影响因子：
4.4
作者：
D. Anderson;L. Miller;F. Schmalstieg;R. Rothlein;T. Springer
通讯作者：
D. Anderson;L. Miller;F. Schmalstieg;R. Rothlein;T. Springer
影响因子：
4.4
作者：
S. Shappell;C. Toman;D. Anderson;A. Taylor;M. Entman;C. Smith
通讯作者：
S. Shappell;C. Toman;D. Anderson;A. Taylor;M. Entman;C. Smith
DOI：
10.1016/s0021-9258(18)47151-7
发表时间：
1994-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
T. Kamata;Yoshikazu Takada
通讯作者：
T. Kamata;Yoshikazu Takada
独特的识别位点介导纤维蛋白原与白细胞整合素 Mac-1 (CD11b/CD18) 的相互作用。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Altieri,DC;Agbanyo,FR;Plescia,J;Ginsberg,MH;Edgington,TS;Plow,EF
通讯作者：
Plow,EF
DOI：
--
发表时间：
1994-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Lubing Zhou;D. Lee;J. Plescia;C. Lau;Dario C. Altieri
通讯作者：
Lubing Zhou;D. Lee;J. Plescia;C. Lau;Dario C. Altieri