Reproductive physiological characteristics of tropical Celebes eels Anguilla celebesensis in relation to downstream migration and ovarian development
Reproductive physiological characteristics of tropical Celebes eels Anguilla celebesensis in relation to downstream migration and ovarian development
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DOI：
10.1111/jfb.14231
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发表时间：
2020-01-20
影响因子：
2
通讯作者：
Tsukamoto, Katsumi
中科院分区：
文献类型：
--
作者：
Hagihara, Seishi;Aoyama, Jun;Tsukamoto, Katsumi
从印度尼西亚苏拉威西岛的波索湖-河系统中捕获了64条下游洄游和21条非洄游的雌性西里伯斯鳗（Anguilla celebesensis），并对其生殖生理特性进行了研究。卵巢的组织学观察显示，大多数非洄游鳗鱼处于核周期（43%）或油滴期（48%），而大多数洄游鳗鱼处于卵黄发生早期（36%）或卵黄发生中期（61%）。洄游鳗鲡脑垂体促性腺激素基因（fshb、lhb）的转录水平和血浆性类固醇激素[11-酮睾酮（11-KT）、睾酮、17 β-雌二醇（E-2）]的浓度均显著高于非洄游鳗鲡。fshb信使RNA（m RNA）水平在核仁周期和油滴期较低，在卵黄形成早期显著升高。卵黄发生期鳗鱼的lhb mRNA水平显著高于核周期和油滴期鳗鱼。油滴期和卵黄发生期的11-KT含量显著高于核周期。卵黄发生期的E-2水平显著高于核仁周期和油滴期。这些生殖激素的动态变化反映了中华绒螯蟹卵子发生的生理背景。celebesensis的卵母细胞在向下游迁移之前发育得非常好。
Downstream-migrating (n = 64) and non-migrating (n = 21) female Celebes eels Anguilla celebesensis were captured from the Poso Lake-River system on Sulawesi Island, Indonesia, and their reproductive physiological characteristics were examined. A histological observation of the ovaries revealed that most non-migrating eels were at the perinucleolus (43%) or oil-droplet (48%) stage, whereas most migrating eels were at the early vitellogenic (36%) or midvitellogenic (61%) stage. Transcript levels of gonadotropin genes (fshb, lhb) in the pituitary gland and concentrations of sex steroids [11-ketotestosterone (11-KT), testosterone, 17 beta-oestradiol (E-2)] in blood plasma of migrating eels were significantly higher than those of non-migrating eels. The fshb messenger (m)RNA levels were lower in perinucleolus and oil-droplet stages and then significantly increased in the early vitellogenic stage. The lhb mRNA levels in vitellogenic-stage eels were significantly higher than those in perinucleolus- and oil-droplet-stage eels. The 11-KT levels of eels at the oil-droplet and vitellogenic stages were significantly higher than those of eels at the perinucleolus stage. The E-2 levels at the vitellogenic stage were significantly higher than those at the perinucleolus and oil-droplet stages. These dynamics of the reproductive hormones represented the physiological background of oogenesis in A. celebesensis that has remarkably well-developed oocytes just before downstream migration.