Systematic analysis of the cerebrospinal fluid proteome of fibromyalgia patients
Systematic analysis of the cerebrospinal fluid proteome of fibromyalgia patients
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DOI：
10.1016/j.jprot.2018.04.014
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发表时间：
2019-01-06
影响因子：
3.3
通讯作者：
Kultima, Kim
中科院分区：
文献类型：
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作者：
Khoonsari, Payam Emami;Musunri, Sravani;Kultima, Kim
纤维肌痛(FM)是一种以广泛的肌肉疼痛、疲劳和功能性症状为特征的综合征，由于各种症状与许多其他情况重叠，因此很难诊断。目前，还没有FM的生物标志物，诊断由临床医生主观作出。我们对FM患者和非疼痛对照组的脑脊液(CSF)进行了鸟枪式蛋白质组学研究，以寻找这种综合征的潜在生物标记物候选。基于我们的多变量和单变量分析，我们发现FM患者和对照组的脑脊液蛋白质组的相对差异是中等的。发现了四种重要的蛋白质，用于区分FM患者和非疼痛对照组：载脂蛋白C-III Galectin 3结合蛋白、苹果酸脱氢酶胞浆和神经肽前体蛋白ProSAAS。这些蛋白质参与脂蛋白脂酶(LPL)活性、炎症信号、能量代谢和神经肽信号。意义：纤维肌痛出现在多达2%的人群中，导致肌肉疼痛、僵硬和压痛。根据准确的诊断，非药物和药物治疗可以用来减轻疼痛和控制其他症状。然而，缺乏客观、普遍适用的诊断标准以及模糊和弥漫的症状，使诊断变得困难。在此背景下，我们的发现可以揭示脑脊液蛋白质组在客观诊断FM方面的潜在价值。
Fibromyalgia (FM) is a syndrome characterized by widespread muscular pain, fatigue and functional symptoms, which is known to be difficult to diagnose as the various symptoms overlap with many other conditions. Currently, there are no biomarkers for FM, and the diagnosis is made subjectively by the clinicians. We have performed shotgun proteomics on cerebrospinal fluid (CSF) from FM patients and non-pain controls to find potential biomarker candidates for this syndrome. Based on our multivariate and univariate analyses, we found that the relative differences in the CSF proteome between FM patients and controls were moderate. Four proteins, important to discriminate FM patients from non-pain controls, were found: Apolipoprotein C-III Galectin 3-binding protein, Malate dehydrogenase cytoplasmic and the neuropeptide precursor protein ProSAAS. These proteins are involved in lipoprotein lipase (LPL) activity, inflammatory signaling, energy metabolism and neuropeptide signaling.Significance: Fibromyalgia is present in as much as 2% of the population, causing pain, stiffness, and tenderness of the muscles. Upon accurate diagnostic, nonpharmacological and pharmacological therapies can be used to alleviate pain and manage other symptoms. However, lack of objective, universal applicable diagnostic criteria as well as vague and diffused symptoms, have made diagnosis difficult. In this context, our findings can shed light on potential value of CSF proteome for objectively diagnosing FM.