The effect of a short-term hypocaloric diet on liver gene expression and metabolic risk factors in obese women
The effect of a short-term hypocaloric diet on liver gene expression and metabolic risk factors in obese women
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DOI：
10.1016/j.numecd.2008.06.009
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发表时间：
2009-03-01
影响因子：
3.9
通讯作者：
Kesaniemi, Y.
中科院分区：
文献类型：
--
作者：
Hietaniemi, M.;Jokela, M.;Kesaniemi, Y.
背景和目的：大多数研究肥胖和减肥影响的基因表达研究都是在脂肪组织中进行的。然而，肝脏在维持能量平衡方面也起着核心作用。我们想研究低热量饮食对肝脏整体基因表达和代谢危险因素的影响。方法与结果：研究对象为中年肥胖女性。饮食干预组（n = 12）采用低热量、低脂饮食，持续8周，每日能量摄入5.0 MJ（1200千卡），对照组（n = 19）维持体重。在胆囊手术期间，在饮食期结束时进行肝脏活检。通过比较每组四名受试者的基因表达谱，利用微阵列分析肝脏基因表达。饮食干预组有142个基因表达下调，只有1个基因表达上调。这种饮食导致体重平均下降5%。饮食后甘油三酯和空腹胰岛素浓度显著下降。结论：肝脏基因表达的全球下降是出乎意料的，但结果很有趣，因为它们包括了几个以前与体重减轻无关的基因。然而，由于比较是在体重减轻之后进行的，除了体重变化之外，其他因素也可能参与了两组之间基因表达的差异。甘油三酯和空腹血浆胰岛素浓度的降低与之前的体重研究结果一致。(C) 2008 Elsevier B.V.版权所有
Background and aims: Most gene expression studies examining the effect of obesity and weight loss have been performed using adipose tissue. However, the liver also plays a central role in maintaining energy balance. We wanted to study the effects of a hypocaloric diet on overall hepatic gene expression and metabolic risk factors.Methods and results: The study subjects were middle-aged, obese women. The diet intervention subjects (n = 12) were on a hypocaloric, tow-fat diet for 8 weeks with a daily energy intake of 5.0 MJ (1200 kcal), white the control subjects (n = 19) maintained their weight. Liver biopsies were taken at the end of the diet period during a gallbladder operation. Hepatic gene expression was analyzed using microarrays by comparing the gene expression profiles from four subjects per group. A global decrease in gene expression was observed with 142 down-regulated genes and only one up-regulated gene in the diet intervention group. The diet resulted in a mean weight toss of 5% of body weight. Triglyceride and fasting insulin concentrations decreased significantly after the diet.Conclusions: The global decrease in hepatic gene expression was unexpected but the results are interesting, since they included several genes not previously linked to weight reduction. However, since the comparison was made only after the weight reduction, other factors in addition to weight toss may also have been involved in the differences in gene expression between the groups. The decrease in triglyceride and fasting plasma insulin concentrations is in accordance with results from previous weight-toss studies. (C) 2008 Elsevier B.V. All rights reserved.