Metabolites from the mushroom Ganoderma lingzhi as stimulators of neural stem cell proliferation
Metabolites from the mushroom Ganoderma lingzhi as stimulators of neural stem cell proliferation
复制标题
蘑菇灵芝的代谢物作为神经干细胞增殖的刺激剂
DOI：
10.1016/j.phytochem.2015.03.013
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发表时间：
2015-06-01
期刊：
影响因子：
3.8
通讯作者：
Cheng, Yong-Xian
中科院分区：
文献类型：
--
作者：
Yan, Yong-Ming;Wang, Xin-Long;Cheng, Yong-Xian
灵芝是一种名贵的食药两用真菌，广泛用于防治多种疾病。在本研究中，从该真菌的子实体中分离得到4个螺环[苯并呋喃-2，1‘-环戊烷]结构的硫代萜类化合物，6个环系结构不同的硫代萜类化合物，以及2个已知化合物。用光谱学、X射线结晶学和计算方法确定了这些物质的结构和立体化学。用手性高效液相色谱分离了从真菌中分离得到的7个化合物的(-)-和(+)-对映体。一些代谢物被发现促进神经干细胞(NSCs)的增殖，因此，它们构成了一类NSC刺激物。通过5-溴-2-脱氧尿苷(BrdU)掺入实验表明，该系列中最有效的成员(-)-螺环素A可以影响NSC的细胞周期进程。(C)2015爱思唯尔有限公司。保留所有权利。
Ganoderma lingzhi is a valuable, edible and medicinal fungus that has been widely used for the prevention and treatment of a broad range of diseases. In this study, spirolingzhines A-D, four meroterpenoids with a spiro[benzofuran-2,1'-cyclopentanel motif, lingzhines A-F, six meroterpenoids with diverse ring systems, along with two known compounds were isolated from the fruiting bodies of this fungus. The structures and stereochemistry of these substances were determined by using spectroscopic, X-ray crystallographic and computational methods. Chiral HPLC was used to separate (-)- and (+)-antipodes of seven of ten meroterpenoids, which were isolated from the fungus as racemic mixtures. Several of the metabolites were found to promote proliferation of neural stem cells (NSCs) and, as such, they constitute a class of NSC stimulators. The most potent member of this series, (-)-spirolingzhine A, was shown to affect NSC cell cycle progression using the 5-bromo-2-deoxyuridine (BrdU) incorporation assay. (C) 2015 Elsevier Ltd. All rights reserved.