Induction of an outer membrane protein of 78 kDa in Vibrio vulnificus cultured in the presence of desferrioxamine B under iron-limiting conditions
Induction of an outer membrane protein of 78 kDa in Vibrio vulnificus cultured in the presence of desferrioxamine B under iron-limiting conditions
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DOI：
10.1111/j.1574-6968.2002.tb11246.x
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发表时间：
2002-06-18
影响因子：
2.1
通讯作者：
Yamamoto, S
中科院分区：
文献类型：
--
作者：
Aso, H;Miyoshi, S;Yamamoto, S
虽然已知创伤弧菌能够利用铁胺B作为铁源，但其外膜受体仍有待确定。在这项研究中，我们发现，创伤弧菌表达一种新的外膜蛋白的78 kDa时，生长在去铁胺B的存在下，铁限制条件下。仅在表达该蛋白的细菌细胞中观察到去铁胺B依赖的铁摄取。此外，非变性聚丙烯酰胺凝胶电泳，然后由放射自显影的外膜制备含有78 kDa的蛋白预孵育与[Fe-55]ferrioxamine B提供了一个单一的放射性带，其中的78 kDa的外膜蛋白是目前的主要成分。这些证据表明，可诱导的78-kDa蛋白质可能作为细胞表面受体的ferrioxamine B。(C)2002年由Elsevier Science B. V.代表欧洲微生物学会联合会出版。
Although Vibrio vulnificus is known to be able to utilize ferrioxamine B as an iron source, its outer membrane receptor remains to be determined. In this study, we found that V. vulnificus expressed a new outer membrane protein of 78 kDa when grown in the presence of desferrioxamine B under iron-limiting conditions. The desferrioxamine B-dependent iron uptake was only observed in bacterial cells expressing this protein. Furthermore, non-denaturing polyacrylamide gel electrophoresis followed by autoradiography of the outer membrane preparation containing the 78-kDa protein preincubated with [Fe-55]ferrioxamine B provided a single radioactive band in which the 78-kDa outer membrane protein was present as the major component. These lines of evidence suggest that the inducible 78-kDa protein may serve as the cell-surface receptor for ferrioxamine B. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.