Ultrastructural changes in hepatocellular carcinoma cells induced by exponential pulses of nanosecond duration delivered via a transmission line
Ultrastructural changes in hepatocellular carcinoma cells induced by exponential pulses of nanosecond duration delivered via a transmission line
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DOI：
10.1016/j.bioelechem.2020.107548
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发表时间：
2020-10-01
影响因子：
5
通讯作者：
Zheng, Shusen
中科院分区：
文献类型：
--
作者：
Yin, Shengyong;Liu, Zhen;Zheng, Shusen
高强度脉冲电场的临床应用已被证明可用于消融实体肿瘤。然而，迫切需要开发有效的肿瘤消融装置的新想法。在这里，我们研究了纳秒指数脉冲的细胞效应，这是由电容器放电电路产生的，并通过传输线提供。由传输线振荡升压的峰值电压脉冲具有高的诱导细胞肿胀和导致细胞活力丧失的能力。选择合适的脉冲参数，以研究肿胀细胞的超微结构变化，其呈现平滑的质膜，微绒毛的损失，和降低的细胞质电子密度。我们提出了等效力场假说来理解脉冲诱导细胞肿胀的机制。质膜上的皱纹可能表明细胞肿胀的恢复，并且这通过脉冲PKH 26-细胞与假处理PKH 67-细胞的共培养来验证。结果表明，电脉冲对质膜上带电分子的作用，是导致质膜上形成不规则孔隙等超微结构变化的主要原因。(C)2020爱思唯尔B. V.保留所有权利。
Clinical applications of high-intensity pulsed electric fields have proven useful in ablating solid tumors. However, novel ideas for the development of an effective tumor ablation device are urgently needed. Here, we studied cellular effects of the nanosecond exponential pulse, which is generated by a capacitor-discharging circuit and delivered via a transmission line. Pulses of peak voltage boosted by transmission line oscillation possess high capability to induce swelling and to cause loss of viability in cells. The appropriate parameter of the pulse was selected to investigate the ultrastructural changes in swollen cells, which present smoothened plasma membrane, loss of microvilli, and lowered cytoplasm electron density. We propose the equivalent force field hypothesis to understand the mechanism underlying cell swelling induced by pulsing. Wrinkles on the plasma membrane might indicate recovery from cell swelling, and this was verified by co-culture of pulsed PKH26-Cells with sham-treated PKH67-Cells. We concluded that the ultrastructural changes, such as irregular pores formed on the plasma membrane, were mainly induced by the effect of electric pulse applied on the charged molecules in the membrane. (C) 2020 Elsevier B.V. All rights reserved.