A Method for Cryogenic Preservation of Human Biopsy Specimens and Subsequent Organoid Culture.
A Method for Cryogenic Preservation of Human Biopsy Specimens and Subsequent Organoid Culture.
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DOI：
10.1016/j.jcmgh.2018.04.008
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发表时间：
2018
影响因子：
7.2
通讯作者：
Spence JR
中科院分区：
文献类型：
--
作者：
Tsai YH;Czerwinski M;Wu A;Dame MK;Attili D;Hill E;Colacino JA;Nowacki LM;Shroyer NF;Higgins PDR;Kao JY;Spence JR
人类组织来源的胃肠道（GI）类器官已经彻底改变了人类生物学的研究，1，2，3并且是研究人类生理学和疾病的强大工具;然而，类器官的产生受到人类组织的限制，并且人类样本的存活时间窗口很短，对患者样本可用于研究的时间和地点施加了严格的限制。4这些限制意味着实验室必须在地理上相对靠近采集源，才能在可行性窗口内使用样本。患者来源的类器官也被用于药物开发，干细胞疗法和个性化医疗;然而，考虑到所涉及的时间和劳动力，前瞻性地开发类器官系并不总是可行的。为了克服这些限制，我们试图开发一种实用的方法来冷冻保存活的人类活检组织，然后可以将其冷冻储存或运输，然后解冻以产生仅GI上皮的类器官（也称为肠样/结肠样）的新培养物。在这里，我们描述了一种简单而强大的方法来冷冻保存人类活检标本，随后可以解冻并用于生成仅含上皮的类器官。
Human tissue–derived gastrointestinal (GI) organoids have revolutionized the study of human biology, 1, 2, 3 and are powerful tools for studying human physiology and disease; however, generation of organoids is limited by access to human tissue and a short window of viability for human samples, putting a hard limit on the time and place in which a patient sample can be used for research. 4 These restraints mean that a laboratory must be relatively geographically close to the source of collection to use the sample within the window of viability. Patient-derived organoids also are being used for drug development, stem cell therapies, and personalized medicine; however, it is not always feasible to prospectively develop organoid line efforts given the time and labor involved. 5, 6To overcome these limitations, we sought to develop a practical method to cryopreserve live human biopsy tissue, which then could be stored or shipped frozen and later thawed to generate new cultures of GI epithelium-only organoids (also referred to as enteroids/colonoids). Here, we describe a simple and robust method to cryopreserve human biopsy specimens that subsequently could be thawed and used to generate epithelium-only organoids.
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影响因子：
48
作者：
Yin, Xiaolei;Farin, Henner F.;van Es, Johan H.;Clevers, Hans;Langer, Robert;Karp, Jeffrey M.
通讯作者：
Karp, Jeffrey M.
影响因子：
64.8
作者：
Spence, Jason R.;Mayhew, Christopher N.;Rankin, Scott A.;Kuhar, Matthew F.;Vallance, Jefferson E.;Tolle, Kathryn;Hoskins, Elizabeth E.;Kalinichenko, Vladimir V.;Wells, Susanne I.;Zorn, Aaron M.;Shroyer, Noah F.;Wells, James M.
通讯作者：
Wells, James M.
影响因子：
12.3
作者：
Love MI;Huber W;Anders S
通讯作者：
Anders S
影响因子：
3.6
作者：
Fuller, Megan K.;Faulk, Denver M.;Henning, Susan J.
通讯作者：
Henning, Susan J.
影响因子：
46.9
作者：
Watanabe, Kiichi;Ueno, Morio;Sasai, Yoshiki
通讯作者：
Sasai, Yoshiki