Experimental evaluation of the pathogenicity of Perkinsus olseni in juvenile Manila clams Ruditapes philippinarum.
Experimental evaluation of the pathogenicity of Perkinsus olseni in juvenile Manila clams Ruditapes philippinarum.
复制标题
奥尔森氏菌对菲律宾蛤蜊幼虫致病性的实验评价。
DOI：
10.1016/j.jip.2010.08.007
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发表时间：
2010
影响因子：
3.4
通讯作者：
K. Ogawa
中科院分区：
文献类型：
--
作者：
Jun Shimokawa;T. Yoshinaga;K. Ogawa
采用实验攻毒法对马尼拉蛤（Ruditapes philippinarum）的致病性进行了评价。为了制备奥氏假单胞菌的前动物孢子囊，我们给未感染的马尼拉蛤注射了奥氏假单胞菌纯种的细胞，并饲养了7d。在Ray’s液体巯基乙酸盐培养基中培养后，从注射的蛤的软组织中分离出动物前孢子管。在孵卵场饲养的未感染的幼蛤（壳长3-10mm）中浸泡两种浓度的奥氏单胞菌悬浮液6天。在两种浓度下，攻毒蛤的死亡率都明显高于未攻毒蛤。在较高和较低浓度下，感染剂量依赖性死亡分别在攻击后约4周和7周开始。这是第一个实验证据，证明奥氏单胞菌导致马尼拉蛤直接死亡。估计感染的致死水平约为107个病原体细胞/g软组织重量。
We evaluated the pathogenicity of Perkinsus olseni towards the Manila clam, Ruditapes philippinarum, by an experimental challenge. For production of prezoosporangia of P. olseni, we injected uninfected Manila clams with cells of a pure strain of P. olseni and reared them for 7d. Prezoosporangia were isolated from the soft tissue of the injected clams after culturing in Ray’s fluid thioglycollate medium. Hatchery-reared, uninfected juvenile clams (3–10mm shell length) were challenged by immersion in one of two concentrations of a prezoosporangial suspension of P. olseni for 6d. The challenged clams had significantly higher mortality at both the concentrations than the unchallenged clams. The mortality due to infection dose-dependently began approximately 4weeks and 7weeks after challenge in the higher and lower concentrations, respectively. This is the first experimental evidence that P. olseni causes direct mortality in Manila clams. The lethal level of infection was estimated at approximately 107pathogen cells/g soft tissue weight.