Characterization of end4+, a gene required for endocytosis in Schizosaccharomyces pombe
Characterization of end4+, a gene required for endocytosis in Schizosaccharomyces pombe
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DOI：
10.1002/yea.1134
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发表时间：
2004-07-30
期刊：
影响因子：
2.6
通讯作者：
Takegawa, K
中科院分区：
文献类型：
--
作者：
Iwaki, T;Tanaka, N;Takegawa, K
为了了解裂殖酵母的胞内转运，我们构建了一个end4中断突变体。End4(+)基因编码一种与Sla2p/End4p同源的蛋白质，对酿酒酵母细胞骨架和内吞作用的组装和功能是必不可少的。我们鉴定了裂解酵母突变体end4Delta和ypt7Delta，后者缺乏空泡融合，因此缺乏内吞作用。End 4突变体抑制了Fm4-向液泡膜的转运、荧光黄CH的积累和质膜蛋白Map3-GFP的内化。End4缺失导致与F-肌动蛋白去极化一致的多效性表型，包括高温敏感性、异常形态和交配缺陷。在ypt7突变体中检测到大量的羧基肽酶Y的错误分选，而在end4突变体中检测到很少的错误分选。这些结果表明，在分裂酵母中，End4p在内化过程中是必不可少的，Ypt7p在内吞和空泡蛋白分选途径相交后的内化后影响内吞作用。版权所有(C)2004 John Wiley Sons，Ltd.
To understand endocytic trafficking in Schizosaccharomyces pombe, we constructed an end4 disruption mutant. The end4(+) gene encodes a protein homologous to Sla2p/End4p, which is essential for the assembly and function of the cytoskeleton and endocytosis in Saccharomyces cerevisiae. We characterized the fission yeast mutant end4Delta as well as ypt7Delta, which is deficient in vacuolar fusion and, hence, endocytosis. The delivery of FM4-64 to the vacuolar membrane, accumulation of Lucifer yellow CH and internalization of plasma membrane protein Map3-GFP were inhibited in the end4 mutant. Deletion of end4 resulted in pleiotropic phenotypes consistent with F-actin depolarization, including high temperature sensitivity, abnormal morphology and mating defects. Extensive missorting of carboxypeptidase Y was detected in the ypt7 mutant; however, little missorting was detected in the end4 mutant. These results indicate that End4p is essential for the internalization process and Ypt7p affects endocytosis at a post-internalization step after the intersection of the endocytic and the vacuolar protein-sorting pathways in fission yeast. Copyright (C) 2004 John Wiley Sons, Ltd.