Microwave bioeffects in the erythrocyte are temperature and pO2 dependent: cation permeability and protein shedding occur at the membrane phase transition.
Microwave bioeffects in the erythrocyte are temperature and pO2 dependent: cation permeability and protein shedding occur at the membrane phase transition.
复制标题
红细胞中的微波生物效应取决于温度和 pO2：阳离子渗透性和蛋白质脱落发生在膜相变时。
DOI：
10.1002/bem.2250050215
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发表时间：
1984
影响因子：
1.9
通讯作者：
Penn,A
中科院分区：
文献类型：
--
作者：
Liburdy,RP;Penn,A
微波（2450 MHz, 60 mW/g， CW）暴露兔红细胞仅在膜相变温度（Tc）为17-19°C时增加Na被动转运。这种渗透性效应在细胞内氧张力（pO2≥5 mm Hg）为特征的相对缺氧时增强。在温度匹配（±0.05°C）的假暴露对照组中，渗透率和po2效应均未观察到。此外，在Tc，微波暴露被观察到诱导两种红细胞蛋白的脱落或释放，而在假暴露的对照组中未见。此外，在微波处理的样品中检测到至少7种其他分子量为28,000 D的蛋白质的增强脱落。使用灵敏的银染色，我们估计每个红细胞约有450 fg的蛋白质脱落。这些结果表明温度和po2是影响细胞膜对微波辐射功能和结构响应的重要因素。
Microwave exposure (2450 MHz, 60 mW/g, CW) of rabbit erythrocytes increases Na passive transport only at membrane phase transition temperatures (Tc) of 17–19°C. This permeability effect is enhanced for relative hypoxia which is characteristic of intracellular oxygen tension (pO2⩽ 5 mm Hg). Neither the permeability nor the pO2effects are observed in temperature‐matched (± 0.05°C), sham‐exposed controls. In addition, at Tc, microwave exposure is observed to induce the shedding or release of two erythrocyte proteins not seen in sham‐exposed controls. Moreover, the enhanced shedding of at least seven other proteins all of molecular weight ⩽ 28,000 D was detected in the microwave‐treated samples. Using sensitive silver staining we estimate that approximately 450 fg of protein were shed per erythrocyte. These results demonstrate that temperature and pO2are important influences on both functional and structural responses of cell membranes to microwave radiation.