Higher prevalence and viral load of TT virus in saliva than in the corresponding serum: Another possible transmission route and replication site of TT virus
Higher prevalence and viral load of TT virus in saliva than in the corresponding serum: Another possible transmission route and replication site of TT virus
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DOI：
10.1002/1096-9071(200012)62:4
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发表时间：
2000-12-01
影响因子：
12.7
通讯作者：
Akahane, Y
中科院分区：
文献类型：
--
作者：
Deng, XW;Terunuma, H;Akahane, Y
虽然TT病毒（TTV）可通过血液或血液制品传播，但许多没有输血史的患者已被证明受到感染，这表明其他可能的传播途径。为探讨TTV的传播途径和复制位点，应用半巢式聚合酶链反应技术对85对唾液和血清标本进行了研究。唾液和血清中TTVDNA的检出率分别为38%（32/85）和21%（18/85）。15例患者唾液和血清中均检出TTV DNA。15名患者中有6名患者唾液中的病毒滴度显著高于血清，但血清中的病毒滴度均高于唾液。结果表明，12例患者的唾液和血清中有相同的基因型/亚型，同源性为96-100%。另外3例唾液和血清中存在不同的基因型/亚型，同源性为61.9- 87.2%。在唾液和血清中均观察到混合感染。TTV是否具有唾液嗜性尚需进一步研究。TTV在唾液中的高流行率和病毒载量表明唾液可能是TTV的一种可能传播途径，并且TTV不仅可以在肝组织中复制，还可以在其他组织如口咽组织和/或唾液腺中复制。医学病毒学杂志62：537-537，2000. (C)2000 Wiley-Liss，Inc.
Although TT virus (TTV) is transmissible by blood or blood products, many patients with no history of transfusion of blood and blood products have been shown to be infected, suggesting other possible routes of transmission. To investigate the transmission routes and replication sites of TTV, 85 paired saliva and serum samples were studied by semi-nested polymerase chain reaction. The prevalence of TTV DNA was 38% (32/85 samples) and 21% (18/85) in saliva and serum, respectively. Fifteen patients had TTV DNA both in saliva and serum. Six out of fifteen patients had significantly higher viral titers in saliva than in serum, but none had higher titer in serum than in saliva. When the 222 base-pair nucleotide sequences of PCR products amplified from the samples were analyzed, 12 patients had the same genotype/subtype in saliva and serum and exhibited high homology (96-100%). The other 3 had different genotypes/subtypes in saliva and serum, and the homology was 61.9-87.2%. Mixed infection was observed both in saliva and serum. Further studies are required to determine if a subgroup of TTV has tropism to saliva. The high prevalence and viral load of TTV in saliva suggest that salivary fluid may be a possible route of transmission of TTV and that TTV might replicate not only in liver tissue but also in other tissues such as oropharyngeal tissues and/or salivary glands. J. Med. Virol. 62: 537-537, 2000. (C) 2000 Wiley-Liss, Inc.