Direct allelic variation scanning of the yeast genome
Direct allelic variation scanning of the yeast genome
复制标题
DOI：
10.1126/science.281.5380.1194
复制
发表时间：
1998-08-21
期刊：
影响因子：
56.9
通讯作者：
Davis, RW
中科院分区：
文献类型：
--
作者：
Winzeler, EA;Richards, DR;Davis, RW
随着越来越多的基因组被测序。识别和描述物种内遗传变异的原因将变得越来越重要。事实证明，无需等位基因特异性聚合酶链反应，即可直接有效地扫描、绘制图谱和评分一个物种的任何两个分离株的等位基因变异。无需创建新的菌株或构建体，也不知道变异的具体性质。通过分析两种不同酵母菌株的总基因组 DNA 与高密度寡核苷酸阵列杂交时获得的模式，鉴定出总共 3714 个双等位标记，间隔约 3.5 KB。然后使用这些标记以高分辨率（11 至 64 KB）同时绘制一个多重耐药基因座和四个其他基因座。
As more genomes are sequenced. the identification and characterization of the causes of heritable variation within a species will be increasingly important. It is demonstrated that allelic variation in any two isolates of a species can be scanned, mapped, and scored directly and efficiently without allele-specific polymerase chain reaction. without creating new strains or constructs, and without knowing the specific nature of the variation. A total of 3714 biallelic markers, spaced about every 3.5 kilobases, were identified by analyzing the patterns obtained when total genomic DNA from two different strains of yeast was hybridized to high-density oligonucleotide arrays. The markers were then used to simultaneously map a multidrug-resistance locus and four other Loci with high resolution (11 to 64 kilobases).