Measles virus-specific immunoglobulin G subclass response in serum and cerebrospinal fluid
Measles virus-specific immunoglobulin G subclass response in serum and cerebrospinal fluid
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DOI：
10.1016/s0928-0197(97)10007-1
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发表时间：
1997-11-01
期刊：
影响因子：
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通讯作者：
Kikuta, H
中科院分区：
文献类型：
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作者：
Narita, M;Yamada, S;Kikuta, H
背景资料：以往的研究主要集中在麻疹病毒感染对机体细胞免疫功能的影响上，但目前关于麻疹病毒感染时特异性IgG亚类反应的研究较少。目的：从病毒特异性IgG亚类的角度分析麻疹病毒感染（G1和G3）研究设计：回顾性检测三组患者的冻存血清和/或脑脊液样本;第1组包括14名麻疹初次感染患者，第2组包括10名再感染/疫苗失败患者，第3组包括14名麻疹初次感染患者，7例亚急性硬化性全脑炎患者。使用的方法是改良的ELISA方法，其利用Enclavernost IgG检测试剂盒和小鼠单克隆抗体（Ige 1的克隆HP 6091和IgG 3的克隆HP 6050）。用8 M尿素变性法对所选样本进行了各亚类IgG的亲合力检测。结果：在第1组中，从皮疹发作起7天内可在血清中检测到IgG 3，其频率高于IgG 1。在组2的情况下，在非常急性期血清样品中检测到两个亚类。在这些病例中，IgG I特异性亲和力总是高于IgG 3，在第3组中，脑脊液中检测到的IgG亚类的亲和力低于血清中的IgG亚类。我们的研究结果表明，麻疹病毒感染，像其他病毒感染，IgG 3反应通常发生在IgG 1反应之前，在初次感染的急性期免疫中起主要作用，而IgG 1在免疫维持中起主要作用。在亚急性硬化性全脑炎病例中，中枢神经系统中持续产生的IgG 1和IgG 3可能来自于与血液中不同的细胞群。(C)1997年Elsevier Science B.V.
Background: While many previous studies have focused on the impairment in the cellular immunity during measles virus infection, to date, a limited amount of data is available concerning the virus-specific IgG subclass response during measles virus infection.Objective: The purpose of this study is to analyze the measles virus infection on the basis of virus-specific IgG subclass (G 1 and G 3).Study design: Frozen-stored, serum and/or cerebospinal fluid samples from three groups of patients were tested retrospectively; Group 1 comprised 14 patients with measles primary infection, group 2, ten patients with reinfection/vaccine failure, and group 3, seven patients with subacute sclerosing panencephalitis. The method used was a modified ELISA method utilizing the Enzygnost IgG detection kit with mouse-monoclonal antibodies (clone HP6091 for Ige 1 and clone HP6050 for IgG 3). Avidity testing for each subclass IgG was also performed for selected samples by means of an 8 M urea-denaturation method,Results: In group 1, the IgG 3 could be detected in serum within 7 days from the onset of rash more frequently than IgG 1. In the cases of group 2, both subclasses were detected in very acute phase serum samples. In these cases, the IgG I-specific avidity was always higher than that of IgG 3, In group 3, the subclass IgGs detected in the cerebrospinal fluid had a lower avidity than those in the serum.Conclusions: Our results suggested that in measles virus infection, like other viral infections, the IgG 3 response normally occurs before the IgG 1 response, and plays a major role in the acute phase immunity during the primary infection, while the IgG 1 plays a major role in the maintenance of immunity. Continuously produced IgG 1 and IgG 3 in the central nervous system in cases of subacute sclerosing panencephalitis may be derived from cell populations different from those in the blood. (C) 1997 Elsevier Science B.V.