Orthodontic Root Resorption was Associated with the Secretion of IL-6 and IL-8 Stimulated by IL-17in Dental Pulp Cells
Orthodontic Root Resorption was Associated with the Secretion of IL-6 and IL-8 Stimulated by IL-17in Dental Pulp Cells
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DOI：
10.5466/ijoms.11.172
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
Mami Shimizu;M. Yamaguchi;M. Asano;S. Fujita;T. Utsunomiya;Hirotsugu Yamamoto;K. Kasai
中科院分区：
文献类型：
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作者：
Mami Shimizu;M. Yamaguchi;M. Asano;S. Fujita;T. Utsunomiya;Hirotsugu Yamamoto;K. Kasai
正畸诱导的炎症性牙根吸收是正畸牙齿移动的常见并发症。本研究旨在探讨正畸牙移动过程中牙髓对牙根吸收的影响。15只8周龄雄性BALB/c小鼠在25 g的过度正畸力作用下，使其上颌第一磨牙向近中倾斜，持续9天。通过免疫组织化学分析确定牙髓（DP）中TRAP、白细胞介素（IL）-17、IL-17受体（IL-17 R）、IL-6和角质形成细胞趋化因子（KC;啮齿动物中的IL-8相关蛋白）蛋白的表达水平。此外，IL-17对IL-6和IL-8的产生的影响进行了研究，使用人牙髓细胞（hDP）在体外。在体实验牙移动研究中，25 g组观察到多核细胞的吸收陷窝。IL-17，IL-17 R，IL-6和KC的免疫反应性检测在所有的DP组织进行的正畸力在第九天。此外，IL-17增加hDP细胞释放IL-6和IL-8。提示IL-17可能通过增加hDP细胞IL-6和IL-8的产生而加重牙根吸收过程。
Orthodontically induced inflammatory root resorption (OIIRR) is a common complication associated with orthodontic tooth movement. The aim of this study was to investigate how dental pulp contributes to root resorption during orthodontic tooth movement. Fifteen male 8-week-old BALB/c mice were subjected to an excessive orthodontic force of 25g to induce a mesially tipping movement of the upper first molars for nine days. The expression levels of the TRAP, interleukin(IL)-17, IL-17 receptor(IL-17R), IL-6 and keratinocyte chemoattractant (KC; IL-8-related protein in rodents) proteins were determined in dental pulp (DP) by an immunohistochemical analysis. Furthermore, the effects of IL-17 on the production of IL-6 and IL-8 were investigated using human dental pulp (hDP) cells in vitro. Resorption lacunae with multinucleated cells were observed in the 25g group during the in vivo experimental tooth movement study. Immunoreactivity for IL-17, IL-17R, IL-6 and KC was detected in all of the DP tissues subjected to the orthodontic force on day nine. Moreover, IL-17 increased the release of IL-6 and IL-8 from hDP cells. The results of this study suggest that the IL-17 may aggravate the process of root resorption by increasing the production of IL-6 and IL-8 from hDP cells.