A R2/R1 ratiometric procedure for a concentration-independent, pH-responsive, Gd(III)-based MRI agent
A R2/R1 ratiometric procedure for a concentration-independent, pH-responsive, Gd(III)-based MRI agent
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DOI：
10.1021/ja062387x
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发表时间：
2006-09-06
影响因子：
15
通讯作者：
Terreno, Enzo
中科院分区：
文献类型：
--
作者：
Aime, Silvio;Fedeli, Franco;Terreno, Enzo
一种新的程序，使pH响应性的一个基于gd的探针独立于其浓度已经开发。该方法基于比率法，需要在高于1 T的磁场下测量顺磁对水质子纵向和横向核磁共振弛豫率的贡献，并在纳秒时间尺度上使用具有旋转迁移率的基于gd的探针。该方法已在体外通过使用基于多鸟氨酸骨架的大分子加合物进行了测试和验证，其旋转迁移率取决于pH值。本通讯报道的结果大大提高了MRI反应剂的诊断潜力。
A novel procedure for making the pH responsiveness of a Gd-based probe independent of its concentration has been developed. The method is based on a ratiometric approach and requires the measurement, at magnetic fields higher than 1 T, of the paramagnetic contribution to the longitudinal and the transverse NMR relaxation rates of water protons in the presence of a Gd-based probe with a rotational mobility in the nanosecond time scale. The method has been tested and validated in vitro by using a macromolecular adduct based on the polyornithine backbone, whose rotational mobility is pH dependent. The results reported in this communication improve substantially the diagnostic potential of MRI responsive agents.