Molecualr characterization, expression, polymorphism of NR5A2 and its relationship with litter size in Hu sheep
Molecualr characterization, expression, polymorphism of NR5A2 and its relationship with litter size in Hu sheep
复制标题
湖羊NR5A2分子特征、表达、多态性及其与产羔数的关系
DOI：
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发表时间：
2015
影响因子：
0.4
通讯作者：
Qifa Li
中科院分区：
文献类型：
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作者：
Yinxia Li;Jun Zhang;Yong Qian;Chunhua Meng;Huili Wang;Xiaojing Tao;Sheng Zhong;Shaoxian Cao;Qifa Li
NR 5A 2与哺乳动物的类固醇生成、细胞增殖、卵巢卵泡发育、排卵和生育力等多种过程有关。然而，关于NR 5A 2与哺乳动物多产性之间关系的数据缺乏。本研究对湖羊NR 5A 2基因进行了鉴定和分析，并研究了NR 5A 2基因与湖羊繁殖性能的相关性。该基因编码区全长1488 bp，在哺乳动物中具有保守性。湖羊卵巢NR 5A 2 mRNA水平与排卵率和产羔数呈正相关。我们检测到NR 5A 2编码序列中的两个单核苷酸多态性（T40 C和T1419 C）。在第3胎和平均胎次，T40 C位点CC基因型的胡氏母羊的产羔数大于TT或TC基因型的;在T1419 C位点，TT基因型的胡氏母羊的产羔数大于CC基因型的。研究结果表明，NR 5A 2基因与湖羊繁殖性能相关。
NR5A2 has been implicated in processes as diverse as steroidogenesis, cellular proliferation, ovarian follicular development, ovulation, and fertility in mammals. However, data about the relationship between NR5A2 and prolificacy in mammals are lacking. In the present study, we identified and characterized NR5A2 of Hu sheep, and investigated the correlation beween NR5A2 and reproductive performance. The full-length coding region was 1488 bp, and the gene was conserved in mammals. We found a positive correlation between NR5A2 mRNA levels int he ovary and the ovulation rate and litter size of Hu sheep. we detected two single nucleotide polymorphisms(T40C and T1419C) in the coding sequence of NR5A2. At the third and average parity, litter size of Hu ewes with CC genotype at T40C locus was larger than those of ewes with TT or TC genotypes; at the T1419C locus, Hu ewes with TT genotype was greater than those of ewes with CC genotype at the third parity. Our findings demonstrated that NR5A2 was associated with reproductive performance in Hu sheep, a high prolificacy breed.