Genomic profiling of gastric carcinoma in situ and adenomas by array-based comparative genomic hybridization
Genomic profiling of gastric carcinoma in situ and adenomas by array-based comparative genomic hybridization
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DOI：
10.1002/path.2686
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发表时间：
2010-05-01
影响因子：
7.3
通讯作者：
Moriyama, Masatsugu
中科院分区：
文献类型：
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作者：
Uchida, Masahiro;Tsukamoto, Yoshiyuki;Moriyama, Masatsugu
虽然基因组拷贝数畸变（CNAs）在晚期胃癌已经被广泛的阵列比较基因组杂交（阵列CGH）分析的特点，在原位胃癌（CIS）仍然知之甚少。此外，没有报道表明CNA和胃腺瘤的组织病理学特征之间的相关性。在这项研究中，我们研究了20个胃CIS（维也纳分类4.2）和20个腺瘤，包括7个低级别腺瘤（LGA;维也纳分类3）和13个高级别腺瘤（HGA;维也纳分类4.1）的CNA，使用基于谷胱甘肽的阵列CGH。CIS中最常见的畸变是8 q（85%）和20 q（500/0）的增加，以及5 q（50%）和17 p（500/0）的丢失，表明这些CNA参与了CIS的发展。我们发现HGA中CNA的模式与LGA中CNA的模式有很大不同。HGA中最常见的CNA是8 q（62%）和7 pq（54%）的增加，而LGA中最常见的CNA是7q21.3-q22.1（57%）的增加和5 q（43%）的丢失。有趣的是，在HGA中最常见的8 q和7 pq的增益在任何LGA病例中都没有检测到。值得注意的是，在HGA和CIS中最常检测到8 q增益，但在LGA中未检测到。由于HGA被认为比LGA具有更高的进展为浸润性癌的风险，这些数据表明8 q增加对于胃腺瘤的恶性转化是重要的。版权所有（C）2010大不列颠和爱尔兰病理学会。由John Wiley & Sons有限公司出版
Although genomic copy number aberrations (CNAs) of gastric carcinoma at the advanced stage have already been extensively characterized by array comparative genomic hybridization (array CGH) analysis, those of gastric carcinoma in situ (CIS) are still poorly understood. Furthermore, no reports have demonstrated correlations between CNAs and histopathological features of gastric adenoma. In this study, we investigated CNAs of 20 gastric CISs (Vienna category 4.2) and 20 adenomas including seven low-grade adenomas (LGA; Vienna category 3) and 13 high-grade adenomas (HGA; Vienna category 4.1), using oligonucleotide-based array CGH. The most frequent aberrations in CIS were gains at 8q (85%) and 20q (500/0), and losses at 5q (50%) and 17p (500/0), suggesting that these CNAs are involved in the development of CIS. We found that the pattern of CNAs in HGA was quite different from that in LGA. The most frequent CNAs in HGA were gains at 8q (62%) and 7pq (54%), whereas those in LGA were gain at 7q21.3-q22.1 (57%) and loss at 5q (43%). Interestingly, gains at 8q and 7pq, both of which occurred most frequently in HGA, were not detected in any cases of LGA. Of note, 8q gain was detected most frequently in both HGA and CIS but was undetected in LGA. Since HGA is believed to have a higher risk of progression to invasive carcinoma than LGA, these data suggest that 8q gain is important for the malignant transformation of gastric adenoma. Copyright (C) 2010 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.