Molecular identification of new picornaviruses and characterization of a proposed enterovirus 73 serotype
Molecular identification of new picornaviruses and characterization of a proposed enterovirus 73 serotype
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DOI：
10.1099/0022-1317-82-2-409
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发表时间：
2001-02-01
影响因子：
3.8
通讯作者：
Pallansch, MA
中科院分区：
文献类型：
--
作者：
Oberste, MS;Schnurr, D;Pallansch, MA
肠道病毒（EV）传统上通过在病毒中和试验中使用病毒型特异性抗血清来鉴定。1955年、1964年和1978年在美国加州分离的3株EV毒株和1995年在阿曼分离的1株EV毒株被发现在抗原性上彼此相关;然而，这些菌株不能被标准EV分型抗血清中和，表明它们可能代表一种新的EV血清型，通过RT-PCR结合扩增子测序和与肠道病毒核苷酸序列数据库的比较来表征分离株的遗传特征，这些毒株的VP 1全序列同源性为75.3 ~ 87.2%，但与任何EV血清型的原型毒株的同源性不超过68%，它们的衣壳全序列彼此密切相关，但与任何EV血清型的原型毒株的衣壳全序列仅相距较远，加州和阿曼分离株与EV簇群B的成员关系最密切，表明它们是未分类的成员确定了一个分离株CA 55 -1988的全基因组序列，预测的多聚蛋白序列与其它B群EV的多聚蛋白序列的同源性为86.5 ~ 89.2%，与其它群EV的多聚蛋白序列的同源性为56.7 ~ 61.9%。从两大洲40多年的样品中分离到这种新的EV血清型表明它在广泛的地理区域内持续传播。为了与标准的小核糖核酸病毒命名法保持一致，我们建议将这种新的血清型命名为“肠道病毒73型”（EV 73）。
Enteroviruses (EV) have traditionally been identified by using serotype-specific antisera in a virus-neutralization test. Three EV strains isolated in California, USA, in 1955, 1964 and 1978, and a 1995 Oman isolate, were found to be antigenically related to one another; however, the strains were not neutralized by standard EV typing antisera, suggesting that they may represent a new EV serotype, The isolates were characterized genetically by RT-PCR coupled with amplicon sequencing and comparison to a database of enterovirus nucleotide sequences, The strains were 75.3 to 87.2% identical to one another in complete VP1 nucleotide sequence, but no more than 68% identical in sequence to the prototype strain of any EV serotype, Their complete capsid sequences were closely related to one another, but only distantly related to those of any EV prototype strain, The California and Oman isolates were most closely related to members of EV cluster B, suggesting that they are unclassified members (i.e. a new serotype) of cluster B, The complete genome sequence was determined for one isolate, CA55-1988, and the predicted polyprotein sequence was 86.5 to 89.2% identical to those of other cluster B EV and 56.7 to 61.9% identical to the polyprotein sequences of EV belonging to other clusters. Isolation of this new EV serotype from samples obtained on two continents and over a period of 40 years suggests continued circulation over a wide geographical area, In keeping with standard picornavirus nomenclature, we propose that this new serotype be named 'enterovirus 73' (EV73).