Antimicrobial efficacy of 2.5% sodium hypochlorite, 2% chlorhexidine, and ozonated water as irrigants in mesiobuccal root canals with severe curvature of mandibular molars.
Antimicrobial efficacy of 2.5% sodium hypochlorite, 2% chlorhexidine, and ozonated water as irrigants in mesiobuccal root canals with severe curvature of mandibular molars.
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DOI：
10.4103/ejd.ejd_324_17
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发表时间：
2018-01
影响因子：
--
通讯作者：
Campos FU
中科院分区：
文献类型：
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作者：
Pinheiro SL;Silva CCD;Silva LAD;Cicotti MP;Bueno CEDS;Fontana CE;Pagrion LR;Dalmora NP;Daque TT;Campos FU
本研究的目的是评价2.5%次氯酸钠、2%洗必泰和臭水对下颌磨牙弯曲严重的近中颊根管中粪肠球菌、变形链球菌和白色念珠菌生物膜的抑菌效果。这是一项在微生物学实验室进行的体外实验研究。用粪肠球菌、变形链球菌、白色念珠菌等标准菌株对60个下颌磨牙弯曲严重的近中颊根管进行污染。根据灌注液的不同随机分为四组(n=15)：SH：2.5%次氯酸钠；CH：2%洗必泰；臭氧：臭氧；对照：双蒸水。用WAVEONE Gold一次往复系统预备近颊根管。在根管的冠状1/3、中1/3和根尖1/3分别进行了3个周期的短进短出刷牙。在第一个周期之前使用了Proglider文件。统计分析采用单因素方差分析和Tukey的多重比较检验。在仪器安装前后分别采集样本进行活菌计数。灌流后各组生物膜均显著减少(P<；0.01)。检测后，次氯酸钠(98.07%)、洗必泰(98.31%)和臭氧水(98.02%)的细菌总数与双蒸水(对照，72.98%)相比显著减少(P<；0.01)。在这项研究中测试的所有灌水剂都显示出类似的抗菌活性。因此，臭氧水可能是根管系统中微生物减少的一种选择。
The aim of this study is to evaluate the antimicrobial efficacy of 2.5% sodium hypochlorite, 2% chlorhexidine, and ozonated water on biofilms of Enterococcus faecalis, Streptococcus mutans, and Candida albicans in mesiobuccal root canals with severe curvature of mandibular molars. This was an experimental ex vivo study in microbiologic laboratory. Sixty mesiobuccal root canals with severe curvature of mandibular molars were contaminated with standard strains of E. faecalis, S. mutans, and C. albicans. The specimens were randomly divided into four groups (n = 15) according to irrigating solution: SH: 2.5% sodium hypochlorite; CH: 2% chlorhexidine; O3: ozonated water; and control: double-distilled water. The mesiobuccal root canals of all groups were instrumented with the WaveOne Gold Primary reciprocating system. Three cycles of instrumentation with three short in-and-out brushing motions were performed: (1) in the coronal third, (2) in the middle third, and (3) in the apical third of the canal. A ProGlider file was used before the first cycle. Statistical analysis was performed using one-way analysis of variance followed by Tukey's multiple comparison test. Samples were collected for viable bacterial counts before and after instrumentation. All groups showed significant biofilm reduction after irrigation (P < 0.01). After instrumentation, sodium hypochlorite (98.07%), chlorhexidine (98.31%), and ozonated water (98.02%) produced a significantly reduction in bacterial counts compared with double-distilled water (control, 72.98%) (P < 0.01). All irrigants tested in this study showed similar antimicrobial activity. Thus, ozonated water may be an option for microbial reduction in the root canal system.