An enzyme cocktail for efficient protoplast formation in Aspergillus niger
An enzyme cocktail for efficient protoplast formation in Aspergillus niger
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DOI：
10.1016/j.mimet.2008.11.001
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发表时间：
2009-03-01
影响因子：
2.2
通讯作者：
Wosten, Han A. B.
中科院分区：
文献类型：
--
作者：
de Bekker, Charissa;Wiebenga, Ad;Wosten, Han A. B.
Novozym 234传统上用于制备用于真菌遗传转化的原生质体。由于它不再在市场上，一种新的酶鸡尾酒被定义为原生质体黑曲霉。该鸡尾酒由哈茨木霉的裂解酶、灰链霉菌的几丁质酶和梨形螺旋体的葡糖苷酶组成。(C) 2008 Elsevier B.V.版权所有
Novozym 234 has been traditionally used to prepare protoplasts for genetic transformation of fungi. Since it is no longer on the market, a new enzyme cocktail was defined to protoplast Aspergillus niger. The cocktail consists of lysing enzymes from Trichoderma harzianum, chitinase from Streptomyces griseus and beta-glucuronidase from Helix pomatia. (C) 2008 Elsevier B.V. All rights reserved.