DADA2: High-resolution sample inference from Illumina amplicon data.
DADA2: High-resolution sample inference from Illumina amplicon data.
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DOI：
10.1038/nmeth.3869
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发表时间：
2016-07
期刊：
影响因子：
48
通讯作者：
Holmes SP
中科院分区：
文献类型：
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作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
我们提出了DADA 2，一个软件包，模型和纠正Illumina测序扩增子错误。DADA 2可以准确地推断样本序列，而无需粗粒化为OTU，并解决小至一个核苷酸的差异。在几个模拟社区中，DADA 2比其他方法识别出更多的真实的变体，并且输出更少的假序列。我们将DADA 2应用于一组孕妇的阴道样本，揭示了以前未检测到的卷曲乳杆菌变体的多样性。
We present DADA2, a software package that models and corrects Illumina-sequenced amplicon errors. DADA2 infers sample sequences exactly, without coarse-graining into OTUs, and resolves differences of as little as one nucleotide. In several mock communities DADA2 identified more real variants and output fewer spurious sequences than other methods. We applied DADA2 to vaginal samples from a cohort of pregnant women, revealing a diversity of previously undetected Lactobacillus crispatus variants.