Iliac and mandible osteoblasts exhibit varied responses to LMHF vibration
Iliac and mandible osteoblasts exhibit varied responses to LMHF vibration
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DOI：
10.1002/cbin.11019
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发表时间：
2018-10-01
影响因子：
3.9
通讯作者：
Leethanakul, Chidchanok
中科院分区：
文献类型：
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作者：
Pravitharangul, Anute;Suttapreyasri, Srisurang;Leethanakul, Chidchanok
面骨和长骨具有不同的发育起源、结构和细胞组成。本研究旨在比较体外培养的人下颌骨和长骨成骨细胞对低强度高频机械振动（LMHF）的骨重建介质表达的反应。从三个人的髂嵴和下颌骨标本中制备成骨细胞样细胞培养物，并在成骨诱导培养基中培养。通过每3天分析DNA含量、碱性磷酸酶活性和基因表达，持续27天，证实成熟成骨细胞表型的诱导。基于基因表达，诱导培养第15天形成成熟的成骨细胞。在诱导培养基中培养15天后，使成熟成骨细胞每24小时经受振动（0、30或60 Hz）30分钟。48 h后，通过总细胞mRNA的实时PCR分析和细胞上清液的ELISA测定RANKL、OPG、IL-1、IL-6和TGF-β基因和蛋白的表达。LMHF振动对髂骨和下颌骨成骨细胞均产生了反应：IL-1和RANKL mRNA表达下调，IL-6 mRNA表达上调。然而，在髂骨成骨细胞中TGF-β mRNA不变，OPG mRNA上调，而在下颌骨成骨细胞中TGF-β和OPG mRNA均下调。结果，LMHF降低了髂骨成骨细胞中RANKL/OPG mRNA的比例，但未改变下颌骨成骨细胞中RANKL/OPG mRNA的比例。本研究表明，成熟的髂骨成骨细胞对振动具有更强的抗吸收反应，而成熟的下颌骨成骨细胞对振动的抗吸收反应不明显。
The facial and long bones have distinct developmental origins, structures, and cellular compositions. This study aimed to compare the in vitro responses of human mandible and long bone osteoblasts to low-magnitude, high-frequency (LMHF) mechanical vibration in terms of expression of mediators of bone remodeling. Osteoblast-like cell cultures were prepared from iliac crest and mandibular bone specimens from three individuals and cultured in osteogenic induction media. Induction of mature osteoblastic phenotypes was confirmed by analysis of DNA content, alkaline phosphatase activity and gene expression every 3 days for 27 days. Based on gene expression, mature osteoblasts formed by day 15 of induction culture. After 15 days of culture in induction media, mature osteoblasts were subjected to vibration (0, 30, or 60Hz) for 30min every 24h. After 48h, RANKL, OPG, IL-1, IL-6 and TGF- gene, and protein expression were determined by real-time PCR analysis of total cellular mRNA and ELISAs of the cell supernatants. Both iliac and mandible osteoblasts responded to LMHF vibration: IL-1 and RANKL mRNA were downregulated and IL-6 mRNA was upregulated. However, TGF- mRNA was unaltered and OPG mRNA was upregulated in iliac osteoblasts, whereas both TGF- and OPG mRNA were downregulated in mandible osteoblasts. As a result, LMHF reduced the RANKL/OPG mRNA ratio in iliac osteoblasts but did not alter the RANKL/OPG mRNA ratio in mandible osteoblasts. This study suggests mature iliac osteoblasts exhibit a more potent anti-resorptive response to vibration, while this tendency was not obviously apparent in mature mandible osteoblasts.