In vivo time-course imaging of tumor angiogenesis in colorectal liver metastases in the same living mice using two-photon laser scanning microscopy.
In vivo time-course imaging of tumor angiogenesis in colorectal liver metastases in the same living mice using two-photon laser scanning microscopy.
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DOI：
10.1155/2012/265487
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发表时间：
2012
影响因子：
--
通讯作者：
Kusunoki M
中科院分区：
文献类型：
--
作者：
Tanaka K;Morimoto Y;Toiyama Y;Matsushita K;Kawamura M;Koike Y;Okugawa Y;Inoue Y;Uchida K;Araki T;Mizoguchi A;Kusunoki M
在体内实时可视化的过程中的血管生成在继发性肿瘤在同一活的动物提出了一个重大的挑战，转移研究。我们开发了一种使用双光子激光扫描显微镜（TPLSM）在活体小鼠中进行结直肠肝转移发展的活体成像技术。我们还开发了时间序列TPLSM，其中在几天到几个月的时间内进行了多次活体TPLSM程序。将表达红色荧光蛋白的结直肠癌细胞接种到表达绿色荧光蛋白的小鼠的脾脏中。第一轮和第二轮活体TPLSM允许肝窦或Disse间隙中活癌细胞（红色）的可视化。第三轮活体TPLSM显示肝转移集落由活的癌细胞和具有肿瘤血管的周围基质组成（绿色）。使用时间序列TPLSM在同一活体小鼠中进行肿瘤血管生成的体内时间过程成像可能是抗血管生成药物评价的理想工具，减少了个体间差异的影响。
In vivo real-time visualization of the process of angiogenesis in secondary tumors in the same living animals presents a major challenge in metastasis research. We developed a technique for intravital imaging of colorectal liver metastasis development in live mice using two-photon laser scanning microscopy (TPLSM). We also developed time-series TPLSM in which intravital TPLSM procedures were performed several times over periods of days to months. Red fluorescent protein-expressing colorectal cancer cells were inoculated into the spleens of green fluorescent protein-expressing mice. First- and second-round intravital TPLSM allowed visualization of viable cancer cells (red) in hepatic sinusoids or the space of Disse. Third-round intravital TPLSM demonstrated liver metastatic colonies consisting of viable cancer cells and surrounding stroma with tumor vessels (green). In vivo time-course imaging of tumor angiogenesis in the same living mice using time-series TPLSM could be an ideal tool for antiangiogenic drug evaluation, reducing the effects of interindividual variation.