Architecture of peroxisomal alcohol oxidase crystals from the methylotrophic yeast Hansenula polymorpha as deduced by electron microscopy
Architecture of peroxisomal alcohol oxidase crystals from the methylotrophic yeast Hansenula polymorpha as deduced by electron microscopy
复制标题
通过电子显微镜推断甲基营养酵母汉逊酵母的过氧化物酶体醇氧化酶晶体的结构
DOI：
--
复制
发表时间：
1992
影响因子：
3.2
通讯作者：
E. van Bruggen
中科院分区：
文献类型：
--
作者：
J. Vonck;E. van Bruggen
根据体外生长的多态汉逊酵母类似晶体的电子显微镜照片，推测了体内存在于甲醇营养酵母过氧化物体中的乙醇氧化酶晶体的构筑。观察到晶体的三种特征，以及在晶体形成的非常早期阶段的单层。晶体被认为是立方体，每个八聚体分子与四个相邻分子在一个平面上以与其四个轴成直角的方式进行相同的接触。该单位细胞包含六个八聚体，三个相互垂直的方向，和两个大洞，可以容纳其他参与甲醇代谢的过氧化体蛋白。晶体包含连接孔洞的通道，这些孔洞允许相对较大的分子通过晶体扩散。晶体的形成依赖于每个亚单位只有一个接触，这可能解释了晶体的脆弱性。
The architecture of alcohol oxidase crystalloids occurring in vivo in the peroxisomes of methylotrophic yeasts was deduced from electron micrographs of similar crystals of the Hansenula polymorpha enzyme grown in vitro. Three characteristic views of the crystal are observed, as well as single layers in the very early stages of crystal formation. The crystal is concluded to be cubical, with every octameric molecule making the same contacts with four neighbors in one plane, at right angles to its fourfold axis. The unit cell contains six octamers, in three mutually orthogonal orientations, and two large holes, which can accommodate other peroxisomal proteins involved in methanol metabolism. The crystal contains channels, connecting the holes, which allow the diffusion of relatively large molecules through the crystal. Crystal formation depends on just one contact per subunit, which may explain the fragility of the crystals.
二羟基丙酮合酶是博伊丁假丝酵母甲醇诱导的过氧化物酶体的丰富成分。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Goodman,JM
通讯作者：
Goodman,JM
影响因子：
5.6
作者：
MURTHY, MRN;REID, TJ;ROSSMANN, MG
通讯作者：
ROSSMANN, MG
DOI：
10.1016/s0021-9258(17)39756-9
发表时间：
1984-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J M Goodman;C W Scott;P N Donahue;J P Atherton
通讯作者：
J M Goodman;C W Scott;P N Donahue;J P Atherton