Evaluation of a Western Equine Encephalitis recombinant E1 protein for protective immunity and diagnostics
Evaluation of a Western Equine Encephalitis recombinant E1 protein for protective immunity and diagnostics
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DOI：
10.1016/j.antiviral.2004.06.008
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发表时间：
2004-11-01
影响因子：
7.6
通讯作者：
Suresh, MR
中科院分区：
文献类型：
--
作者：
Das, D;Gares, SL;Suresh, MR
西方马脑炎（WEE）的E1和E2糖蛋白是WEE亚单位疫苗开发的候选抗原。我们克隆了WEE病毒的E1基因，并在大肠杆菌中以包涵体形式表达。包涵体被成功地溶解、重折叠，并在小鼠中评估了这种非糖基化蛋白的免疫原性。用重组E1蛋白免疫小鼠产生体液和细胞介导的免疫应答，表明重组E1蛋白具有免疫原性。用活的WEE病毒攻击E1免疫的小鼠，证明对这种E几乎没有保护作用。大肠杆菌来源的非糖基化亚基。(C)2004 Elsevier B.V.保留所有权利。
The E1 and E2 glycoproteins of Western Equine Encephalitis (WEE) are candidate antigens for WEE subunit vaccine development. We have cloned the E1 gene of WEE virus and expressed it in Escherichia coli as inclusion bodies. The inclusion bodies were successfully solubilised, refolded and the immunogenicity of this unglycosylated protein was assessed in mice. Immunization of mice with recombinant E1 protein generated both humoral and cell-mediated immune responses, indicating the recombinant E1 protein is immunogenic. Challenge of E1-immunized mice with live WEE virus demonstrated little or no protection from this E. coli-derived non-glycosylated subunit. (C) 2004 Elsevier B.V. All rights reserved.