Mitotic catastrophe induced by exposure of V79 Chinese hamster cells to low-energy protons
Mitotic catastrophe induced by exposure of V79 Chinese hamster cells to low-energy protons
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DOI：
10.1080/095530099140050
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发表时间：
1999-06-01
影响因子：
2.6
通讯作者：
Mackey, MA
中科院分区：
文献类型：
--
作者：
Ianzini, F;Cherubini, R;Mackey, MA
目的：测定低能质子照射诱发的有丝分裂灾变率，并与X射线照射进行比较。材料：用0.5、1、2、5、10Gy质子和X射线照射中国仓鼠V79细胞。实验用的质子LET分别为7.7、11.0和30.5keVmum(-1)，能量分别为5.01、3.20和0.76 MeV。结果：质子辐照可增强V79细胞有丝分裂突变的诱导作用。核碎裂是有丝分裂灾难的一个标志，发生在质子粒子照射后的细胞比X射线照射的细胞早得多。结论：V79细胞在质子和X射线照射后的几代细胞中持续发生有丝分裂灾难，但质子比X射线更有效地诱导了这种现象。这些结果从它们在空间暴露中的重要性以及受辐射细胞的后代可能获得基因组不稳定性的角度进行了讨论。
Purpose: To determine the yield of mitotic catastrophe induced after low energy proton irradiation and to compare this yield with that induced by X-rays.Materials: Asynchronous Chinese hamster V79 cells were irradiated with 0.5, 1, 2, 5 and 10 Gy proton beams and X-rays. Proton LET of 7.7, 11.0 and 30.5 keV mu m(-1), corresponding to energies of 5.01, 3.20 and 0.76 MeV respectively, evaluated at the cell mid-plane, were used for experiments. The occurrence and yield of mitotic catastrophe was measured as the percentage of cells exhibiting fragmented nuclei.Results: Proton irradiation led to an enhanced induction of mitotic catastrophe in V79 cells. The onset of nuclear fragmentation, a hallmark of mitotic catastrophe, occurred much earlier after cell exposure to proton particles than to X-ray irradiation.Conclusions: Mitotic catastrophe is persistent in the subsequent cell generations after proton and X irradiation of V79 cells; but protons are more effective than X-rays for the induction of this phenomenon. These results are discussed in terms of their importance in space exposures and possible acquisition of genomic instability by the progeny of irradiated cells.