Effect of fibroblasts on tracheal epithelial regeneration in vitro
Effect of fibroblasts on tracheal epithelial regeneration in vitro
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DOI：
10.1089/ten.2006.12.2619
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发表时间：
2006-09-01
影响因子：
--
通讯作者：
Omori, Koichi
中科院分区：
文献类型：
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作者：
Kobayashi, Ken;Nomoto, Yukio;Omori, Koichi
利用组织工程技术制备了几种人工气管移植物，用于修复缺损气管。本课题组近期将胶原海绵制成的人工气管应用于非环周气管切除术的患者。然而，人工气管表面上的上皮再生缓慢被证实是一个特殊的问题。在这项研究中，我们共同培养气管上皮细胞与成纤维细胞，并检查成纤维细胞对上皮再生的影响在体外。成纤维细胞激活上皮细胞增殖和迁移。在与成纤维细胞共培养时，上皮细胞重建假复层上皮，其由纤毛细胞、杯状细胞和基底细胞组成。此外，在上皮细胞和成纤维细胞之间重建了基底膜，并且在那里也观察到整合素β 4。成纤维细胞迅速增加上皮细胞的粘蛋白分泌。这些结果表明，成纤维细胞对上皮细胞迁移、增殖和分化的刺激作用将缩短上皮细胞覆盖管腔表面缺损所需的时间，并加速形态和功能正常化的上皮的再生，包括基底膜的重建。
Several artificial grafts for covering deficient trachea have been produced through tissue engineering. Recently, our group clinically used an artificial trachea made from collagen sponge for patients with noncircumferential tracheal resection. However, the slowness of epithelial regeneration on the surface of the artificial trachea was confirmed as one particular problem. In this study, we co-cultured tracheal epithelial cells with fibroblasts and examined effects of fibroblasts on epithelial regeneration in vitro. Fibroblasts activated epithelial cell proliferation and migration. In co-culture with fibroblasts, epithelial cells reconstructed pseudostratified epithelium, which was composed of ciliated, goblet, and basal cells. Furthermore, a basement membrane was reconstructed between epithelial cells and fibroblasts, and integrin beta 4 was also observed there. Fibroblasts rapidly increased mucin secretion by epithelial cells. These results indicate that stimulatory effects of fibroblasts on epithelial cell migration, proliferation, and differentiation would reduce the time required for covering of epithelial cells on the defect of luminal surface and hasten regeneration of morphologically and functionally normalized epithelium involving the reconstruction of basement membrane.