A MINOR ARGININE TRANSFER-RNA MUTANT LIMITS TRANSLATION PREFERENTIALLY OF A PROTEIN DEPENDENT ON THE COGNATE CODON
A MINOR ARGININE TRANSFER-RNA MUTANT LIMITS TRANSLATION PREFERENTIALLY OF A PROTEIN DEPENDENT ON THE COGNATE CODON
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DOI：
10.1128/jb.172.5.2504-2510.1990
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发表时间：
1990-05-01
影响因子：
3.2
通讯作者：
WALKER, JR
中科院分区：
文献类型：
--
作者：
CHEN, KS;PETERS, TC;WALKER, JR
大肠杆菌argU基因编码一种罕见的精氨酸tRNA（反密码子UCU），它翻译同样罕见的AGA密码子。argU10（Ts）突变是将成熟tRNA的第一个核苷酸从G变为a的转变，可能会破坏受体系统的稳定。当染色体以单倍体状态出现时，该突变在摄氏30度时降低生长速率，在摄氏43度时60 ~ 90分钟后停止生长。该突变还优先限制（与总蛋白合成相比）依赖于五个AGA密码子的诱导基因的翻译，即。lambda。cI抑制基因。另一种诱导蛋白的翻译。不涉及AGA密码子的-半乳糖苷酶被抑制的程度要小得多。染色体argU（Ts）突变也赋予了Pin表型，即宿主丧失了作为P2溶原抑制噬菌体生长的能力。（E. Haggard-Ljungquist， V. Barreiro， R. Calendar， D. M. Kurnit， H. Cheng, gene 85:25- 33,1989）。
The Escherichia coli argU gene encodes a rare arginine tRNA (anticodon UCU) that translates the similarly rare AGA codon. The argU10(Ts) mutation is a transition that changes the first nucleotide of the mature tRNA from G to A, presumably destabilizing the acceptor stem. This mutation, when present in haploid condition in the chromosome, reduces the growth rate at 30.degree.C and results in cessation of growth after 60 to 90 min at 43.degree.C. The mutation also preferentially limits (compared with total protein synthesis) translation of an induced gene that depends on five AGA codons, i.e., the .lambda. cI repressor gene. Translation of another inducible protein, .beta.-galactosidase, which does not involve AGA codons, was inhibited to a much lesser extent. The chromosomal argU(Ts) mutation also confers the Pin phenotype, that is, loss of ability of the host, as a P2 lysogen, to inhibit growth of bacteriophage .lambda., probably the result of reduced translation of the P2 old gene, which contains five AGA codons (E. Haggard-Ljungquist, V. Barreiro, R. Calendar, D. M. Kurnit, and H. Cheng, Gene 85:25-33, 1989).