Micro-mirrors for nanoscale three-dimensional microscopy.
Micro-mirrors for nanoscale three-dimensional microscopy.
复制标题
用于纳米级三维显微镜的微镜。
DOI：
10.1021/nn900188t
复制
发表时间：
2009
期刊：
影响因子：
17.1
通讯作者：
Wikswo,John
中科院分区：
文献类型：
--
作者：
Seale,Kevin;Janetopoulos,Chris;Wikswo,John
A research-grade optical microscope is capable of resolving fine structures in two-dimensional images. However, three-dimensional resolution, or the ability of the microscope to distinguish between objects lying above or below the focal plane from in-focus objects, is not nearly as good as in-plane resolution. In this issue ofACS Nano, McMahonet al.report the use of mirrored pyramidal wells with a conventional microscope for rapid, 3D localization and tracking of nanoparticles. Mirrors have been used in microscopy before, but recent work with MPWs is unique because it enables the rapid determination of thex-,y-, andz-position of freely diffusing nanoparticles and cellular nanostructures with unprecedented speed and spatial accuracy. As inexpensive tools for 3D visualization, mirrored pyramidal wells may prove to be invaluable aids in nanotechnology and engineering of nanomaterials.
