Stress proteins and stress tolerance in an Antarctic, psychrophilic yeast, Candida psychrophila.
Stress proteins and stress tolerance in an Antarctic, psychrophilic yeast, Candida psychrophila.
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DOI：
10.1111/j.1574-6968.1997.tb12569.x
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发表时间：
1997-06
影响因子：
2.1
通讯作者：
M. L. Deegenaars;K. Watson
中科院分区：
文献类型：
--
作者：
M. L. Deegenaars;K. Watson
描述了在南极嗜冷酵母菌念珠菌中获得耐热性、过氧化耐受性和合成热休克蛋白（hsps）的条件。细胞在15℃下生长，在25℃（3小时）下热休克，获得对热（35℃）和过氧化氢（100 mM）的耐受性。在80和110 kDa下观察到新的热休克诱导蛋白，以及hsp 90、70和60的存在。后一种热休克蛋白不具有显著的热休克诱导作用。hsp104的缺失令人感兴趣，据推测，110kda蛋白可能在嗜冷酵母的应激耐受中发挥作用，类似于hsp104在嗜温酵母中的作用。
Conditions are described for the heat shock acquisition of thermotolerance, peroxide tolerance and synthesis of heat shock proteins (hsps) in the Antarctic, psychrophilic yeast Candida psychrophila. Cells grown at 15 degrees C and heat shocked at 25 degrees C (3 h) acquired tolerance to heat (35 degrees C) and hydrogen peroxide (100 mM). Novel heat shock inducible proteins at 80 and 110 kDa were observed as well as the presence of hsp 90, 70 and 60. The latter hsps were not significantly heat shock inducible. The absence of hsp 104 was intriguing and it was speculated that the 110 kDa protein may play a role in stress tolerance in psychrophilic yeasts, similar to that of hsp 104 in mesophilic species.