Downregulating PKC δ provides a PI3K/Akt-independent survival signal that overcomes apoptotic signals generated by c-Src overexpression
Downregulating PKC δ provides a PI3K/Akt-independent survival signal that overcomes apoptotic signals generated by c-Src overexpression
复制标题
下调 PKC δ 提供独立于 PI3K/Akt 的生存信号，克服 c-Src 过表达产生的细胞凋亡信号
作者：
Minghao Zhong;Zhimin Lu;D. A. Foster
过度表达酪氨酸激酶c-Src的3 Y1大鼠成纤维细胞（3 Y1 c-Src细胞）通过下调蛋白激酶C δ（PKC δ）而转化。然而，当3 Y1 c-Src细胞进行血清撤回，他们通过细胞色素c/caspase 9途径进行凋亡。与此相反，父母或v-Src转化的3 Y1细胞进行细胞凋亡时，血清撤出。如果PKC δ表达下调，则血清撤除诱导的3 Y1 c-Src细胞凋亡表型受到抑制。PKC δ下调产生的表观存活信号不依赖于磷脂酰肌醇-3-激酶（PI 3 K）/Akt存活通路。总的来说，这些数据表明（1）c-Src过表达使细胞对凋亡应激敏感，（2）PKC δ的下调提供了一种新的PI 3 K/Akt非依赖性生存信号，能够抑制凋亡信号。
3Y1 rat fibroblasts overexpressing the tyrosine kinase c-Src (3Y1c-Src cells) become transformed by downregulation of protein kinase C δ (PKC δ). However, when 3Y1c-Src cells were subjected to serum withdrawal, they underwent apoptosis via a cytochrome c/caspase 9 pathway. In contrast, neither parental nor v-Src-transformed 3Y1 cells underwent apoptosis when subjected to serum withdrawal. If PKC δ was downregulated, the apoptotic phenotypes induced by serum withdrawal in the 3Y1c-Src cells were suppressed. The apparent survival signal generated by PKC δ downregulation was independent of the phosphatidylinositol-3-kinase (PI3K)/Akt survival pathway. Collectively, these data indicate that (1) c-Src overexpression renders cells sensitive to apoptotic stress, and (2) that downregulation of PKC δ provides a novel PI3K/Akt-independent survival signal capable of suppressing apoptotic signals.
影响因子：
10.5
作者：
Kennedy, SG;Wagner, AJ;Hay, N
通讯作者：
Hay, N