The autodecomposition of radiolabeled human growth hormone.
The autodecomposition of radiolabeled human growth hormone.
复制标题
放射性标记的人类生长激素的自分解。
DOI：
10.1080/01971528608060462
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Amburn,K
中科院分区：
文献类型：
--
作者：
Baumann,G;Amburn,K
采用温和的乳过氧化物酶技术，用125 I标记人生长激素（hGH）。用SephadexG-100柱层析法对该示踪剂的稳定性和降解产物进行了研究。单体125 I-hGH呈指数下降，半衰期为61天。主要放射性降解产物为碘化物，其出现的分数出现率为0.01136/天。次级降解产物是一系列hGH的放射性低聚物，其总体分数率为0.00525/天。所获得的动力学数据应该为放射性碘标记多肽的保存期和再纯化时间表提供指导。
Human growth hormone (hGH) was radiolabeled with125I, using a gentle lactoperoxidase technique. The stability and decomposition products of this tracer were studied by frequent periodic analysis by Sephadex G-100 chromatography on a long column. Monomeric125I-hGH showed an exponential decline, with a half-life of 61 days. The main radioactive degradation product was iodide, which appeared with a fractional appearance rate of 0.01136 per day. Secondary degradation products were a series of radioactive oligomers of hGH, which appeared with an overall fractional rate of 0.00525 per day. The kinetic data obtained should provide guidelines for the shelf- life and repurification schedule of radioiodinated polypeptides.