Optimization of Hydrogenotrophic Denitrification Behavior Using Continuous and Intermittent Hydrogen Gas Supply
Optimization of Hydrogenotrophic Denitrification Behavior Using Continuous and Intermittent Hydrogen Gas Supply
复制标题
使用连续和间歇氢气供应优化氢营养反硝化行为
DOI：
10.2965/jwet.16-059
复制
发表时间：
2017
影响因子：
--
通讯作者：
F. Kazama
中科院分区：
文献类型：
--
作者：
R. Eamrat;Y. Tsutsumi;T. Kamei;W. Khanichaidecha;Yasuhiro Tanaka;F. Kazama
地下水硝酸盐污染已成为影响饮用水质量和人类健康的严重问题。研究了一种高效、低成本、简易的氢营养化反硝化反应器。采用连续供氢（1-15 mL/min）优化氢气（H2）供应。结果表明，最佳工艺条件为5 mL/min，脱氮效率为86.4%，氢气效率为199 mg-N/g-H2。在后续的实验中，为了提高氢气效率和耗氢量，采用了间歇式供氢。采用短周期补氢循环（补氢3 min，无补氢7 min），脱氮效率达到96.5%，氢气效率提高到744 mg-N/g-H2。此外，属于变形菌门和β变形菌纲的细菌是微生物群落的主要组成部分。连续系统下以hydroophaga spp.（39.3%）为优势种，间歇系统下以Thauera spp.（58.5%）最多。在本研究中，负责HD的hydroophaga spp., Thauera spp.和Rhodocyclaceae对地下水的氮具有有效的去除作用。
Nitrate contamination of groundwater has become a serious issue affecting the quality of drinking water and human health. An energy-efficient, low-cost, and simple reactor was developed to remove nitrate via hydrogenotrophic denitrification (HD). Hydrogen (H2) supply was optimized by using a continuous supply of hydrogen (1–15 mL/min). The results revealed that the optimal condition was 5 mL/min, which yielded a nitrogen removal efficiency of 86.4% and a hydrogen effectiveness of 199 mg-N/g-H2. In the subsequent experiment, an intermittent hydrogen supply was used to improve the hydrogen effectiveness and hydrogen consumption. Using a cycle with a short period of hydrogen supply (3 min with hydrogen supply and 7 min with no hydrogen supply), excellent nitrogen removal efficiency (96.5%) was achieved, and the hydrogen effectiveness increased to 744 mg-N/g-H2. Furthermore, bacteria belonging to the Proteobacteria phylum and Betaproteobacteria class were the major components of the microbial community. However, Hydrogenophaga spp. (39.3%) was dominant under the continuous system, whereas Thauera spp. (58.5%) was the most abundant species under the intermittent system. In this study, Hydrogenophaga spp., Thauera spp., and Rhodocyclaceae, which were responsible for HD, afforded in efficient nitrogen removal from groundwater.