Send Orders of Reprints at Bspsaif@emirates.net.ae Genotype Distribution of Human Papillomaviruses in Japanese Women with Abnormal Cervical Cytology
Send Orders of Reprints at Bspsaif@emirates.net.ae Genotype Distribution of Human Papillomaviruses in Japanese Women with Abnormal Cervical Cytology
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发送重印订单至 Bspsaif@emirates.net.ae 宫颈细胞学异常的日本女性中人乳头瘤病毒的基因型分布
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通讯作者：
I. Kukimoto
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作者：
K. Kondo;Asami Uenoyama;R. Kitagawa;H. Tsunoda;Rika Kusumoto‐Matsuo;S. Mori;Y. Ishii;T. Takeuchi;T. Kanda;I. Kukimoto
我们使用 PGMY-CHUV 检测报告了宫颈细胞学异常的日本女性中人乳头瘤病毒 (HPV) 的患病率和基因型分布，这是一种基于 PGMY-PCR 的线印迹检测，经过验证并显示适合以最小偏差检测样本中的多种 HPV 类型。从 326 名巴氏涂片异常的门诊患者采集的宫颈脱落细胞中提取总 DNA。总体而言，307 份样本 (94%) 为 HPV 阳性，其中 30% 含有多种基因型。意义未明的非典型鳞状细胞 (ASC-US) 中 HPV DNA 的患病率为 83%（49/59 个样本）； 91%（20/22样本）为非典型鳞状细胞，不能排除高级别鳞状上皮内病变（ASC-H）； 97%（130/134 个样本）为低度鳞状上皮内病变 (LSIL)；高级鳞状上皮内病变 (HSIL) 为 99%（85/86 个样本）。 HSIL 中检测到的三种最常见的 HPV 类型是 HPV16 (36%)、HPV52 (24%) 和 HPV58 (14%)。我们的研究结果表明，日本女性中多种 HPV 感染比之前报道的更为普遍，并证实与西方国家报道的相比，HPV52 和 58 在她们的宫颈癌前病变中更为占主导地位。
We report the prevalence and genotype distribution of human papillomaviruses (HPVs) among Japanese women with abnormal cervical cytology using the PGMY-CHUV assay, one of PGMY-PCR-based lineblot assays that was validated and shown to be suitable for the detection of multiple HPV types in a specimen with minimum bias. Total DNA was extracted from cervical exfoliated cells collected from 326 outpatients with abnormal Pap smears. Overall, 307 specimens (94%) were HPV-positive, 30% of which contained multiple genotypes. The prevalence of HPV DNA was 83% (49/59 samples) in atypical squamous cells of undetermined significance (ASC-US); 91% (20/22 samples) in atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion (ASC-H); 97% (130/134 samples) in low-grade squamous intraepithelial lesion (LSIL); and 99% (85/86 samples) in high-grade squamous intraepithelial lesion (HSIL). Three most frequent HPV types detected in HSIL were HPV16 (36%), HPV52 (24%), and HPV58 (14%). Our results suggest that multiple HPV infections are more prevalent in Japanese women than previously reported, and confirm that HPV52 and 58 are more dominant in their cervical precancerous lesions when compared to those reported in Western countries.
影响因子：
10.3
作者：
Castle, PE;Solomon, D;Wheeler, CM
通讯作者：
Wheeler, CM
影响因子：
10.3
作者：
Kjaer, Susanne K.;Frederiksen, Kirsten;Iftner, Thomas
通讯作者：
Iftner, Thomas