Tests of the simple model of Lin and Brandts for the folding kinetics of ribonuclease A.
Tests of the simple model of Lin and Brandts for the folding kinetics of ribonuclease A.
复制标题
对核糖核酸酶 A 折叠动力学的 Lin 和 Brandts 简单模型进行测试。
DOI：
10.1021/bi00310a002
复制
发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Baldwin,RL
中科院分区：
文献类型：
--
作者：
Schmid,FX;Buonocore,MH;Baldwin,RL
Franz X. Schmid、Michael H. Buonocore 和 Robert L. Baldwin* 摘要：L.-N. Lin 和 J. F. Brandts 最近提出了一个简单的核糖核酸酶 A 折叠动力学模型，其中折叠中间体是不可检测的。我们测试了主要未折叠物种的简单模型的基本假设，这些物种是在解折叠后通过缓慢异构化（根据 Lin 和 Brandts 的“X^Y 反应”）产生的。简单模型假设在重折叠过程中，缓慢的 Y-* X 反应必须在任何折叠发生之前发生。我们测量了Y——*·
Franz X. Schmid, Michael H. Buonocore, and Robert L. Baldwin* abstract: L.-N. Lin and J. F. Brandts recently proposed a simple model for the folding kinetics of ribonuclease A in which folding intermediates are not detectable. We have tested the basic assumption of the simple model for the major un-folded species, which is produced by a slow isomerization (the “X^ Y reaction” according to Lin and Brandts) after un-folding. The simple model assumes that in refolding the slow Y-* X reaction must occur before any folding can take place. We have measured the Y—*·