myc boxes, which are conserved in myc family proteins, are signals for protein degradation via the proteasome
myc boxes, which are conserved in myc family proteins, are signals for protein degradation via the proteasome
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DOI：
10.1128/mcb.18.10.5961
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发表时间：
1998-10-01
影响因子：
5.3
通讯作者：
Wright, APH
中科院分区：
文献类型：
--
作者：
Flinn, EM;Busch, CMC;Wright, APH
快速降解的c-myc蛋白的细胞水平在决定细胞的增殖状态中起着重要作用。c-myc水平升高通常与快速增殖的肿瘤细胞有关。我们在这里展示了myc盒I和II，在myc蛋白家族所有成员的N端发现，其功能是指导c-myc蛋白的降解。myc盒I和盒II都包含足够的信息，可以独立指导它们融合的稳定表达蛋白的降解。至少有一部分myc盒定向降解是通过蛋白酶体发生的。myc盒导向降解的机制似乎在酵母和哺乳动物细胞之间是保守的。我们的研究结果表明，myc盒子可能在调节c-myc蛋白的水平和活性方面发挥重要作用。
Cellular levels of the rapidly degraded c-myc protein play an important role in determining the proliferation status of cells. Increased levels of c-myc are frequently associated with rapidly proliferating tumor cells. We show here that myc boxes I and II, found in the N termini of all members of the myc protein family, function to direct the degradation of the c-myc protein. Both myc boxes I and II contain sufficient information to independently direct the degradation of otherwise stably expressed proteins to which they are fused. At least part of the myc box-directed degradation occurs via the proteasome. The mechanism of myc box-directed degradation appears to be conserved between yeast and mammalian cells. Our results suggest that the myc boxes may play an important role in regulating the level and activity of the c-myc protein.