High genetic diversity of the attachment (G) protein of human metapneumovirus
High genetic diversity of the attachment (G) protein of human metapneumovirus
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DOI：
10.1128/jcm.42.8.3406-3414.2004
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发表时间：
2004-08-01
影响因子：
9.4
通讯作者：
Kobayashi, K
中科院分区：
文献类型：
--
作者：
Ishiguro, N;Ebihara, T;Kobayashi, K
对7株新分离的人偏肺病毒(HMPV)的核蛋白(N)、磷蛋白(P)、基质蛋白(M)、融合蛋白(F)、M2-1蛋白、M2-2蛋白、小分子疏水蛋白(SH)和附着蛋白(G)的全长基因进行了分析，并与已发表的hMPV基因进行了比较。核苷酸序列的系统发育分析表明，有两个基因群，暂定为群I和群2，与人类呼吸道合胞病毒的群相似。虽然预测的N、P、M、F和M2氨基酸序列在两组间高度保守(氨基酸同源性，N为96%，P为85%，M为97%，F为94%，M2-1为95%，M2-2为90%)，但SH和G蛋白的氨基酸同源性较低(SH，58%；G，33%)。此外，通过系统发育分析，每个类群可分为两个亚组，暂定为亚组1A和113以及亚组2A和2B。各亚组成员的G氨基酸序列高度保守(氨基酸同源性分别为：A组88%，113组93%，213组96%)。HMPV的G基因被认为是主要的抗原决定簇，在中和抗体的产生中起重要作用。阐明G的抗原多样性对于流行病学分析和制定预防hMPV感染的策略是重要的。
Complete genes encoding the predicted nucleoprotein (N), phosphoprotein (P), matrix protein (M), fusion protein (F), M2-1protein, M2-2protein, small hydrophobic protein (SH), and attachmentprotein (G) of seven newly isolated human metapneumoviruses (hMPVs) were analyzed and compared with previously published data for hMPV genes. Phylogenetic analysis of the nucleotide sequences indicated that there were two genetic groups, tentatively named groups I and 2, similar to the grouping of human respiratory syncytial virus. Although the predicted amino acid sequences of N, P, M, F, and M2 were highly conserved between the two groups (amino acid identities, 96% for N, 85% for P, 97% for M, 94% for F, 95% for M2-1, and 90% for M2-2), the amino acid identities of the SH and G proteins were low (SH, 58%; G, 33%). Furthermore, each group could be subdivided into two subgroups by phylogenetic analysis, tentatively named subgroups 1A and 113 and subgroups 2A and 2B. The predicted amino acid sequences of G within members of each subgroup were highly conserved (amino acid identities, 88% for group 1A, 93% for group 113, and 96% for group 213). The G of hMPV is thought to be the major antigenic determinant and to play an important role in the production of neutralizing antibodies. Clarification of the antigenic diversity of G is important for epidemiological analysis and for establishment of strategies to prevent hMPV infection.