Alterations in the innate immune system due to exhausting exercise in intensively trained rats
Alterations in the innate immune system due to exhausting exercise in intensively trained rats
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DOI：
10.1038/s41598-020-57783-4
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发表时间：
2020-01-22
影响因子：
4.6
通讯作者：
Castell, Margarida
中科院分区：
文献类型：
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作者：
Estruel-Amades, Sheila;Camps-Bossacoma, Mariona;Castell, Margarida
众所周知，密集的体力活动会改变免疫系统的功能。然而，运动强度和持续时间的影响需要更深入的研究。我们的目的是建立在先天免疫反应的变化引起的两个程序的强化训练大鼠相比，久坐不动的大鼠。短期训练计划包括2周的强化训练，以疲劳测试结束（疲劳短期训练，S-TE）。第二个训练计划包括5周的训练，包括每周两次疲劳测试和三次训练。在这种情况下，在额外的最终耗竭测试之前（T）、之后立即（TE）和之后24小时（TE 24）评估免疫状态。对生物标志物如吞噬活性、巨噬细胞细胞因子和活性氧（ROS）产生以及自然杀伤（NK）细胞活性进行定量。S-TE不足以诱导评估的先天免疫生物标志物的变化。然而，第二次训练伴随着吞噬活性的下降，细胞因子分泌模式的变化和ROS产生的巨噬细胞和NK细胞比例减少，但增加NK细胞毒活性。总之，一个5周的高强度训练计划，而不是一个较短的训练，诱导先天免疫系统功能的改变。
It is known that intensive physical activity alters the immune system's functionality. However, the influence of the intensity and duration of exercise needs to be studied in more depth. We aimed to establish the changes in the innate immune response induced by two programmes of intensive training in rats compared to sedentary rats. A short training programme included 2 weeks of intensive training, ending with an exhaustion test (short training with exhaustion, S-TE). A second training programme comprised 5-week training including two exhaustion tests and three trainings per week. In this case, immune status was assessed before (T), immediately after (TE) and 24h after (TE24) an additional final exhaustion test. Biomarkers such as phagocytic activity, macrophage cytokine and reactive oxygen species (ROS) production, and natural killer (NK) cell activity were quantified. S-TE was not enough to induce changes in the assessed innate immunity biomarkers. However, the second training was accompanied by a decrease in the phagocytic activity, changes in the pattern of cytokine secretion and ROS production by macrophages and reduced NK cell proportion but increased NK cytotoxic activity. In conclusion, a 5-week intense training programme, but not a shorter training, induced alterations in the innate immune system functionality.