Intraspecific phylogeography of the gopher tortoise, Gopherus polyphemus: RFLP analysis of amplified mtDNA segments
Intraspecific phylogeography of the gopher tortoise, Gopherus polyphemus: RFLP analysis of amplified mtDNA segments
复制标题
地鼠龟 (Gopherus polyphemus) 的种内系统发育地理学：扩增 mtDNA 片段的 RFLP 分析
作者：
M. F. OSENTOSKI;T. Lamb
海龟线粒体DNA进化的缓慢速度限制了传统的限制性片段调查可检测到的种内变异水平。我们用另一种限制性分析方法检测了地鼠(Gopherus Polyphemus)线粒体DNA的变异，在这种方法中，线粒体基因组的PCR扩增片段被四核苷酸位点的内切酶消化。对代表4个扩增区域的限制性片段多态性进行了分析，以评估整个物种范围内112只乌龟的群体遗传结构。通过UPGMA和简约分析，确定了36个单倍型，并解析了三个主要的地理组合(东部、西部和佛罗里达中部)。东部和西部的组合毗邻阿巴拉契科拉水系，而佛罗里达中部的组合似乎仅限于布鲁克斯维尔山脊。东部和西部的组合界线与来自美国东南部的另外8个物种的系统地理轮廓非常一致，这些物种既代表淡水领域，也代表陆地领域。
The slow rate of mtDNA evolution in turtles poses a limitation on the levels of intraspecific variation detectable by conventional restriction fragment surveys. We examined mtDNA variation in the gopher tortoise (Gopherus polyphemus) using an alternative restriction assay, one in which PCR‐amplified segments of the mitochondrial genome were digested with tetranucleotide‐site endonucleases. Restriction fragment polymorphisms representing four amplified regions were analysed to evaluate population genetic structure among 112 tortoises throughout the species' range. Thirty‐six haplotypes were identified, and three major geographical assemblages (Eastern, Western, and Mid‐Florida) were resolved by UPGMA and parsimony analyses. Eastern and Western assemblages abut near the Apalachicola drainage, whereas the Mid‐Florida assemblage appears restricted to the Brooksville Ridge. The Eastern/Western assemblage boundary is remarkably congruent with phylogeographic profiles for eight additional species from the southeastern U.S., representing both freshwater and terrestrial realms.
影响因子：
3.3
作者：
M. Nei;F. Tajima
通讯作者：
M. Nei;F. Tajima