Up-regulation of vasopressin mRNA in paraventricular hypophysiotrophic neurons after acute immobilization stress.
Up-regulation of vasopressin mRNA in paraventricular hypophysiotrophic neurons after acute immobilization stress.
复制标题
急性固定应激后室旁促生理营养神经元中加压素 mRNA 上调。
作者：
Viktor Bartanusz;Jean;D. Ježová;Judit S. Baffi;J. Z. Kiss
本研究测定了急性束缚应激后大鼠下丘脑室旁核内加压素（VP）和促肾上腺皮质激素释放因子（CRF）mRNA的水平。信使RNA水平测定原位杂交组织化学使用35 S标记的合成DNA寡核苷酸探针和定量放射自显影。与密度值相关的直方图分析显示，制动后2小时，平均细胞VP和CRF mRNA水平显著增加（分别为对照值的145 +/-30.3%和68 +/-21.3%）。此外，VP表达的小细胞神经元的数量增加了一倍，在应激大鼠相对于对照组。这些结果表明，单一的急性制动应激是足以上调VP以及CRF mRNA的生产在下丘脑CRF神经分泌系统。
Cellular levels of vasopressin (VP) and corticotropin-releasing factor (CRF) mRNAs were determined in the rat hypothalamic paraventricular nucleus after acute immobilization stress. Messenger RNA levels were measured by in situ hybridization histochemistry using 35S-labeled synthetic DNA oligonucleotide probes and quantitative autoradiography. Analysis of histograms related to density values revealed that two hours after immobilization the average cellular VP and CRF mRNA levels were significantly increased (145 +/- 30.3 and 68 +/- 21.3%, respectively, above control values). Moreover, the number of VP-expressing parvicellular neurons was doubled in stressed rats relative to controls. These results indicate that single acute immobilization stress is sufficient to up-regulate VP as well as CRF mRNA production in the hypothalamic CRF-neurosecretory system.