Hypoxia Enhances Fusion of Oral Squamous Carcinoma Cells and Epithelial Cells Partly via the Epithelial-Mesenchymal Transition of Epithelial Cells
Hypoxia Enhances Fusion of Oral Squamous Carcinoma Cells and Epithelial Cells Partly via the Epithelial-Mesenchymal Transition of Epithelial Cells
复制标题
缺氧部分通过上皮细胞的上皮-间质转化增强口腔鳞状细胞癌细胞和上皮细胞的融合
DOI：
10.1155/2018/5015203
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发表时间：
2018-01-01
影响因子：
--
通讯作者：
Shang, Zheng-Jun
中科院分区：
文献类型：
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作者：
Huang, Chun-ming;Yan, Ting-lin;Shang, Zheng-Jun
越来越多的证据表明，细胞融合在肿瘤的发生和转移中起着至关重要的作用，而缺氧是与肿瘤微环境密切相关的因素，可导致EMT，从而诱导肿瘤生长过程中的血管生成和转移。然而，缺氧与融合之间的关系尚未有报道。EMT会改变上皮细胞表面的一些蛋白质，细胞表面蛋白质的改变可能会促进细胞融合。本研究发现，低氧可促进口腔鳞癌细胞（OSCC）与人永生化口腔上皮细胞（HIOECs）之间的自发细胞融合。同时缺氧可导致EMT的发生，缺氧预处理的HIOECs与OSCC的融合率增加，而EMT阻断剂DAPT可显著降低HIOECs与OSCC的融合率。因此，上皮细胞可以通过EMT自发地增加与OSCC的细胞融合。我们的研究可能为肿瘤微环境、细胞融合和癌症之间的联系提供新的见解。
Increasing evidence and indications showed that cell fusion is crucial in tumor development and metastasis, and hypoxia, a closely linked factor to tumor microenvironment, which can lead to EMT, induces angiogenesis and metastasis in tumor growth. However, the relationship between hypoxia and fusion has not been reported yet. EMT will change some proteins in the epithelial cell surface and the changes of proteins in cell surface may increase cell fusion. This study found that hypoxia promotes the spontaneous cell fusion between Oral Squamous Carcinoma Cells (OSCCs) and Human Immortalized Oral Epithelial Cells (HIOECs). At the same time, Hypoxia can lead to EMT, and hypoxia-pretreated HIOECs increased fusion rate with OSCC, while the fusion rate was significantly reduced by DAPT, a kind of EMT blocker. Therefore, epithelial cells can increase spontaneously cell fusion with OSCC by EMT. Our study may provide a new insight to link among tumor microenvironment, cell fusion, and cancer.