Analysis of mRNA levels for the MHC class I-like molecules CD1 and FcRn in preimplantation mouse embryos.
Analysis of mRNA levels for the MHC class I-like molecules CD1 and FcRn in preimplantation mouse embryos.
复制标题
植入前小鼠胚胎中 MHC I 类分子 CD1 和 FcRn 的 mRNA 水平分析。
DOI：
10.1111/j.8755-8920.2000.430408.x
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Paschetto,MG
中科院分区：
文献类型：
--
作者：
Warner,CM;Paschetto,MG
问题：着床前胚胎上表达的主要组织相容性复合体（MHC）抗原对于控制胚胎的发育、繁殖和母体对胚胎的异体识别非常重要。基于相似的三维蛋白结构，最近定义了四种MHC I类和MHC I类抗原：Ia类、Ib类、Ic类和Id类。Ia类和Ib类抗原由MHC编码，而Ic类和Id类抗原由其他染色体上的基因编码。Ia类和Ib类MHC抗原均在植入前小鼠胚胎上表达。Ia类抗原对胚胎的作用尚不清楚，但一种Ib类抗原Qa‐2 （pedgene的产物）的功能已被发现控制早期卵裂分裂的速率和随后的胚胎存活。Ic类和Id类抗原在着床前胚胎上的表达尚未得到评价。在本研究中，我们报道了两个类基因，cd1和fcrn，在植入前小鼠胚胎的mRNA表达分析。研究方法：采用逆转录聚合酶链反应（RT - PCR）方法分析C57BL/6小鼠1 -细胞、2 -细胞、8 -细胞和囊胚期胚胎中cd1和fcrnmrna水平。结果所有着床前胚胎均未发现cd1mrna的表达。作为本研究的副产物，我们在已发表的小鼠secd1基因序列中发现了一个错误：cDNA中的核苷酸746是G而不是c。该碱基变化位于限制性内切酶psti识别的位点，从而消除了aPstI的切割位点。fcrnmrna在植入前各阶段均有表达。与1细胞期和囊胚期胚胎相比，2细胞期和8细胞期胚胎中fcrn mRNA水平较高。结论小鼠着床前胚胎中存在fcrnmrna，但不存在cd1 mRNA。
PROBLEMMajor histocompatibility complex (MHC) antigens expressed on preimplantation embryos are important for the control of development, reproduction, and allo‐recognition of the embryo by the mother. Four types of MHC class I and MHC class I‐like antigens have recently been defined: class Ia, class Ib, class Ic, and class Id, based on their similar three‐dimensional protein structures. Class Ia and class Ib antigens are encoded in the MHC, whereas class Ic and class Id antigens are encoded by genes on other chromosomes. Both class Ia and class Ib MHC antigens are expressed on preimplantation mouse embryos. The function of the class Ia antigens on embryos is unknown, but the function of one class Ib antigen, Qa‐2, the product of thePedgene, has been found to control the rate of early cleavage division and subsequent embryo survival. The expression of class Ic and class Id antigens on preimplantation embryos has not yet been evaluated. In the present study, we report the analysis of mRNA expression of two class Id genes,CD1andFcRn, in preimplantation mouse embryos.METHOD OF STUDYA reverse transcription‐polymerase chain reaction (RT‐PCR) assay was performed to analyze mRNA levels forCD1andFcRnin 1‐cell, 2‐cell, 8‐cell, and blastocyst stage embryos from C57BL/6 mice.RESULTSNo expression ofCD1mRNA was found in any of the preimplantation embryos tested. As a by‐product of this study, we found a mistake in the published sequence of the mouseCD1gene: nucleotide 746 in the cDNA is a G not a C. This base change is in a site recognized by the restriction enzymePstI, thereby eliminating aPstI cleavage site. Expression of mRNA forFcRnwas found in all preimplantation stages tested. Higher levels of mRNA forFcRnwere detectable in 2‐cell and 8‐cell embryos compared to 1‐cell and blastocyst stage embryos.CONCLUSIONThis study shows that mRNA forFcRnbut not forCD1is found in preimplantation mouse embryos.