Vaccination with dendritic cells pulsed with homogenate protein of spinal cord promotes functional recovery from spinal cord injury in mice
Vaccination with dendritic cells pulsed with homogenate protein of spinal cord promotes functional recovery from spinal cord injury in mice
复制标题
用脊髓匀浆蛋白脉冲的树突状细胞疫苗接种可促进小鼠脊髓损伤的功能恢复
DOI：
10.1038/sc.2008.112
复制
发表时间：
2009-05-01
期刊：
影响因子：
2.2
通讯作者：
Bian, X.
中科院分区：
文献类型：
--
作者：
Liu, M.;Zhao, J.;Bian, X.
研究设计：将脊髓匀浆蛋白冲击的未成熟树突状细胞(DC)接种于小鼠体内，进行脊髓损伤(SCI)后神经功能恢复的随机双盲动物实验。目的：研究hpDC对脊髓损伤小鼠神经功能恢复的影响。方法：将脊髓匀浆蛋白、髓鞘碱性蛋白(MBP)或磷酸盐缓冲液(PBS)冲击的未成熟树突状细胞(DC)局部或腹膜注射于脊髓损伤小鼠。每周评定脊髓功能恢复情况(Basso、Beattie和Bresna han旷场运动评定量表、BBB评分)。结果：注射后84天，hpDCs组小鼠的BBB评分为18.2±1.1，显著高于mBDC组和对照组(16.3±2.1和10.0±2.0)。HpDCs组的损伤区面积、囊变面积及胶质瘢痕厚度均小于对照组。结论：脊髓匀浆蛋白致敏的树突状细胞局部或腹膜移植对脊髓损伤后功能恢复和神经保护有显著作用。
Study design:Randomized, double-blinded animal experiment for neural functional recovery from spinal cord injury (SCI) through vaccination with immature dendritic cells (DCs) pulsed with homogenate protein of spinal cord (hpDCs) in mice.Objective:To study the effect of hpDCs in the recovery from SCI in mice.Method:Immature DCs pulsed with homogenate protein of spinal cord, myelin basic protein (MBP) or phosphate-buffer solution (PBS) were injected into spinal cord-injured mice locally or peritoneally. The functional recovery of spinal cord (open-field locomotor rating scale of Basso, Beattie and Bresnahan, BBB score) was measured weekly. The areas of injured region and cyst as well as the thickness of the glial scar were measured and the expressions of glial fibrillary acidic protein, neurofilament and nestin were detected to confirm the BBB scores.Results:Eighty-four days after injection, the BBB score of the hpDCs group (peritoneally injected mice) reached 18.2±1.1, significantly higher than that the scores of the mbpDCs and control groups (16.3±2.1 and 10.0±2.0, respectively). The areas of injured region and cyst as well as the thickness of the glial scar of the hpDCs group were less than that of the control group. Meanwhile, the expression of nestin lasts up to 56 days after injection in the hpDCs group, while it disappeared in the mbpDCs and PBS groups.Conclusion:Implanting DCs pulsed with homogenate protein of spinal cord, but not mbpDCs or PBS alone, locally or peritoneally, have a significant effect on functional recovery and neural preservation from SCI.