Electrical detection of TATA binding protein at DNA-modified microelectrodes
Electrical detection of TATA binding protein at DNA-modified microelectrodes
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DOI：
10.1021/ja7106756
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发表时间：
2008-03-12
影响因子：
15
通讯作者：
Barton, Jacqueline K.
中科院分区：
文献类型：
--
作者：
Gorodetsky, Alon A.;Ebrahim, Ali;Barton, Jacqueline K.
一种简单的方法，电化学检测TATA结合蛋白的DNA修饰的微电极上证明。该测定是通用的，并且基于DNA介导的电荷转运至尼罗蓝的中断，尼罗蓝是共价连接至DNA碱基对堆叠的氧化还原活性探针。即使在微摩尔量的牛血清白蛋白、内切核酸酶III或Bam HI甲基转移酶存在下，也可以在微电极上检测到纳摩尔量的TATA结合蛋白。概述的方案提供了一个基础上的敏感的电检测的许多蛋白质在一个单一的DNA芯片。
A simple method for the electrochemical detection of TATA-binding protein is demonstrated at DNA-modified microelectrodes. The assay is general and based on the interruption of DNA-mediated charge transport to Nile Blue, a redox-active probe covalently attached to the DNA base pair stack. Nanomolar quantities of TATA binding protein can be detected on the microelectrodes even in the presence of micromolar amounts of bovine serum albumin, Endonuclease III, or Bam HI methyltransferase. The scheme outlined provides a basis for the sensitive electrical detection of numerous proteins on a single DNA chip.