A Guide to Fluorescent Protein FRET Pairs.
A Guide to Fluorescent Protein FRET Pairs.
复制标题
DOI:
10.3390/s16091488
复制
发表时间:
2016-09-14
期刊:
影响因子:
--
通讯作者:
Chu J
中科院分区:
文献类型:
--
作者:
Bajar BT;Wang ES;Zhang S;Lin MZ;Chu J
Förster or fluorescence resonance energy transfer (FRET) technology and genetically encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live cells with high spatiotemporal resolution. Fluorescent proteins (FPs) are most commonly used as both donor and acceptor fluorophores in FRET biosensors, especially since FPs are genetically encodable and live-cell compatible. In this review, we will provide an overview of methods to measure FRET changes in biological contexts, discuss the palette of FP FRET pairs developed and their relative strengths and weaknesses, and note important factors to consider when using FPs for FRET studies.
登录
查看更多内容
影响因子:
4.6
作者:
Bajar BT;Wang ES;Lam AJ;Kim BB;Jacobs CL;Howe ES;Davidson MW;Lin MZ;Chu J
通讯作者:
Chu J
影响因子:
16.6
作者:
通讯作者:
--
影响因子:
--
作者:
Erard, Marie;Fredj, Asma;Merola, Fabienne
通讯作者:
Merola, Fabienne
DOI:
10.1073/pnas.97.22.11984
发表时间:
2000-10-24
影响因子:
11.1
作者:
Baird, GS;Zacharias, DA;Tsien, RY
通讯作者:
Tsien, RY
影响因子:
4.3
作者:
Betolngar, Dahdjim-Benoit;Erard, Marie;Merola, Fabienne
通讯作者:
Merola, Fabienne