Molecular identification and clinical significance of Mycobacterium seoulense strains from patients with nontuberculous mycobacterium infections
Molecular identification and clinical significance of Mycobacterium seoulense strains from patients with nontuberculous mycobacterium infections
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非结核分枝杆菌感染患者首尔分枝杆菌菌株的分子鉴定及临床意义
DOI：
10.1016/j.diagmicrobio.2022.115756
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Xinling Pan
中科院分区：
文献类型：
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作者：
Jin Zhao;Bin Lu;Yangxiao Zhou;Sipei Wang;Xinling Pan
很少报道M。Hsp65、rpoB和ITS区序列可有效鉴定M. 16SrRNA对M.首尔分枝杆菌（Mycobacterium seoulense，M. seoulense）和物种决定的理想基因。回顾性分析临床症状、实验室检查和放射学检查。对M.对seoulense进行了测序，并构建了分支杆菌菌株的系统发育树。：四个M。根据症状和放射学结果，从4名患者中分离的汉城菌株引起肺部感染。16SrRNA序列分析表明2株菌为M.另外两个为嗜麦芽窄食单胞菌。rpoB、ITS和hsp65序列与M.汉城语。根据ITS、hsp65和rpoB序列构建的系统发育树将4个M. seoulense菌株与M.数据库中的汉城菌株。：M.汉城病毒株可引起人类感染。它们可以通过ITS、hsp65和rpoB基因测序来鉴定。
• Rarely reported M. seoulense strains caused pulmonary infection in four patients • Hsp65, rpoB and ITS region sequences could efficiently identify M. seoulense • Poor power of 16S rRNA in identifying M. seoulense : To investigate the clinical significance of Mycobacterium seoulense ( M. seoulense ) and the ideal gene for species determination. : Clinical symptoms, laboratory examinations, and radiological examinations were retrospectively reviewed. The hsp65 , 16SrRNA, rpoB and ITS region of M. seoulense , were sequenced and phylogenetic trees of mycobacterium strains were constructed. : Four M. seoulense strains isolated from four patients caused pulmonary infections based on the symptoms and radiological results. The 16S rRNA sequence identified two strains as M. intracellulare and the other two as Stenotrophomonas maltophilia . In contrast, the rpoB , ITS, and hsp65 sequences shared high identity with M. seoulense . Notably, the phylogenetic tree based on the ITS, hsp65, and rpoB sequences clustered four M. seoulense strains identified in this study with M. seoulense strains in the database. : M. seoulense strains can cause infection in humans. They can be identified by sequencing ITS, hsp65 , and rpoB genes.