Some γ-motoneurons contain γ-aminobutyric acid in the rat cervical spinal cord
Some γ-motoneurons contain γ-aminobutyric acid in the rat cervical spinal cord
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DOI：
10.1016/j.brainres.2008.01.056
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发表时间：
2008-03-27
期刊：
影响因子：
2.9
通讯作者：
Nojyo, Yoshiaki
中科院分区：
文献类型：
--
作者：
Ito, Tetsufumi;Hioki, Hiroyuki;Nojyo, Yoshiaki
γ-氨基丁酸（GABA）用于外周和中枢神经系统。在这项研究中，纤维免疫反应为67 kDa。谷氨酸脱羧酶（GAD 67）是一种合成GABA的酶，其同工型终止于上肢肌梭的被膜间区域。在C5 ~ T5脊段、臂丛和桡神经的前根中也发现了含GABA的纤维。这些纤维是薄的，胆碱乙酰转移酶（ChAT）的免疫反应。臂丛神经切断后，GABA免疫反应在同侧肱三头肌（TBM）完全消失。荧光金注入后，一些逆行标记的中型神经元GAD 67阳性，但不VGAT mRNA。所有这些观察清楚地表明，下颈脊髓中含有GABA的γ运动神经元将其纤维发送到上肢的肌梭。由于RT-PCR未检测到GABA（A）和GABA（B）受体，因此含GABA的运动神经元的功能尚不清楚。(C)2008 Elsevier B. V.保留所有权利。
gamma-aminobutyric acid (GABA) is utilized in the peripheral as well as central nervous system. In this study, fibers immunoreactive for 67 kDa. isoform of glutamic acid decarboxylase (GAD67), an enzyme which synthesizes GABA, were found to terminate in the intercapsular region of muscle spindles of the upper limb. GABA-containing fibers were also found in the ventral roots of C5 to T5 spinal segments, brachial plexus, and radial nerve. These fibers were thin and immunoreactive for choline-acetyl transferase (ChAT). After transection of the brachial plexus, GABA immunoreactivity disappeared completely in the ipsilateral triceps brachii muscle (TBM). After the injection of fluorogold into the TBM, some retrogradely labeled medium-sized neurons were positive for GAD67, but not VGAT mRNA. All these observations clearly indicate that GABA-containing gamma-motoneurons in the lower cervical spinal cord send their fibers to muscle spindles in the upper extremities. Since we detected neither GABA(A) nor GABA(B) receptors in the TBM by RT-PCR, the function of the GABA-containing gamma-motoneurons remains unclear. (C) 2008 Elsevier B.V. All rights reserved.